Gustafsson B, Huang Y Y, Wigström H
Department of Physiology, University of Göteborg, Sweden.
Neurosci Lett. 1988 Feb 15;85(1):77-81. doi: 10.1016/0304-3940(88)90432-6.
The relationship between the synaptic potentiations evoked by the protein kinase C activator phorbol-12,13-diacetate and by afferent tetanization has been examined in the CA1 region of the hippocampal slice preparation using extracellular recording. It has been found that the potentiation of the field excitatory postsynaptic potential produced by 1 microM phorbol ester does not affect the amount of long-term potentiation (LTP) that can be evoked by afferent tetanization, and vice versa. A dissociation between phorbol ester-induced and tetanus-induced potentiation is also indicated by the fact that only the former was associated with changes in paired-pulse facilitation. On the other hand, as previously described, higher concentrations (10 microM) of phorbol ester blocked the tetanus-induced potentiation. Since the total potentiation given by 10 microM phorbol ester and tetanization depended on the order of presentation of the potentiation-inducing stimuli, it appears that the blockade of LTP is, at least partly, independent of the phorbol ester-induced potentiation.
利用细胞外记录技术，在海马脑片制备的CA1区研究了蛋白激酶C激活剂佛波醇-12,13-二乙酸酯诱发的突触增强与传入纤维强直刺激诱发的突触增强之间的关系。研究发现，1微摩尔佛波醇酯产生的场兴奋性突触后电位增强并不影响传入纤维强直刺激所能诱发的长时程增强（LTP）的量，反之亦然。佛波醇酯诱导的增强和强直刺激诱导的增强之间的分离还表现在，只有前者与双脉冲易化的变化有关。另一方面，如前所述，更高浓度（10微摩尔）的佛波醇酯会阻断强直刺激诱导的增强。由于10微摩尔佛波醇酯和强直刺激产生的总增强取决于增强诱导刺激的呈现顺序，因此看来LTP的阻断至少部分独立于佛波醇酯诱导的增强。
