Kamiya H, Sawada S, Yamamoto C
Department of Physiology, Faculty of Medicine, Kanazawa University, Japan.
Exp Neurol. 1988 Dec;102(3):314-7. doi: 10.1016/0014-4886(88)90225-7.
Application of phorbol esters, selective activators of protein kinase C (PKC), causes a long-lasting enhancement of synaptic transmission in the hippocampus. We examined the relationship between this phorbol ester-induced enhancement of synaptic transmission and long-term potentiation (LTP) quantitatively in the mossy fiber-CA3 synapse of the guinea pig hippocampus in vitro. Potentiating action of 0.5 microM phorbol 12,13-diacetate (PDAc) on synaptic transmission was almost unaffected by preceding tetanic stimuli which had caused a full development of LTP. Tetanic stimulation induced LTP even after synaptic transmission had been enhanced by 0.5 microM PDAc. Quantitative estimations revealed that PDAc-induced synaptic enhancement and LTP were simply additive to each other. The relation between LTP and PKC activation is discussed.
佛波酯是蛋白激酶C（PKC）的选择性激活剂，其应用可使海马体中的突触传递得到持久增强。我们在体外豚鼠海马体的苔藓纤维-CA3突触中，定量研究了这种佛波酯诱导的突触传递增强与长时程增强（LTP）之间的关系。0.5微摩尔佛波醇12,13-二乙酸酯（PDAc）对突触传递的增强作用几乎不受先前已使LTP充分发展的强直刺激的影响。即使在突触传递已被0.5微摩尔PDAc增强后，强直刺激仍可诱导LTP。定量评估表明，PDAc诱导的突触增强和LTP只是简单地相加。文中讨论了LTP与PKC激活之间的关系。
