Pardue H L
Clin Chem. 1977 Dec;23(12):2189-201.
This paper presents a comprehensive classification of kinetic methods of analysis used in clinical chemistry. Objectives of the presentation are to place kinetic methods into perspective with other analytical appraoches, to identify concisely what is and is not a kinetic method, to group different kinetic methods into categories that identify important differences in operational and performance characteristics, and to suggest terminology that can identify the different approaches without ambiguity. In addition to these major objectives, the paper includes a brief historical development of the kinetic approach that covers the period from 1881 to the present, and discusses some current terminology that is believed to be ambiguous and misleading. I suggest that the detection step in analytical methods can be divided into two general categories, called equilibrium and kinetic methods. The kinetic methods are then subdivided into two general categories called fixed sensor-signal and variable sensor-signal methods. Each of these groups is then subdivided into one-point, two-point, and multi-point methods, which are then further subdivided according to what types of blanks are used, what variables are measured, and how the measured data are used to compute enzyme activity or analyte concentration. The paper identifies some commercial instruments that represent different approaches and briefly discusses relative merits of different approaches.
本文介绍了临床化学中使用的动力学分析方法的全面分类。介绍的目的是将动力学方法与其他分析方法进行对比，简明地确定什么是动力学方法，什么不是动力学方法，将不同的动力学方法归类，以识别操作和性能特征方面的重要差异，并提出能够明确识别不同方法的术语。除了这些主要目的外，本文还包括了动力学方法从1881年至今的简要历史发展，并讨论了一些目前认为模糊且具有误导性的术语。我认为分析方法中的检测步骤可分为两大类，即平衡法和动力学法。然后将动力学方法再细分为两大类，即固定传感器信号法和可变传感器信号法。这些类别中的每一类又进一步细分为单点法、两点法和多点法，然后根据所使用的空白类型、测量的变量以及如何使用测量数据来计算酶活性或分析物浓度进行进一步细分。本文确定了一些代表不同方法的商业仪器，并简要讨论了不同方法的相对优点。
