Truchaud A, Glikmanas G, Gourmelin Y, Hersant J, Trepo D, Foray G, Collombel C
Clin Chem. 1985 Sep;31(9):1506-8.
In the disposable rotor of the SAM microcentrifugal analyzer, various lyophilized reagents are predistributed in 24 33-microL cuvets, for determination of multiple analytes in one specimen (e.g., for patient profiles). We evaluated a prototype of this system, which can be used at 25, 30, or 37 degrees C; absorbance readings at 340, 405, and 500 nm varied linearly up to 2.0 A. Starting the reactions by rehydrating the reagents with diluted serum is adequate because absorbance readings do not begin until 180 s after initiating the rehydration. Analytical performances of kinetic determinations at 30 degrees C showed good accuracy and correlation with other methods for creatine kinase, amylase, aspartate aminotransferase, and gamma-glutamyltransferase. Kinetic determinations for urea, and equilibrium determinations with blank corrections for glucose, cholesterol, and triglycerides gave excellent results for glucose and correct results for the other analytes. This compact analyzer combines the analytical performances of a centrifugal analyzer with the practicability of instruments having predistributed reagents.
在SAM微量离心分析仪的一次性转子中，各种冻干试剂预先分布在24个33微升的比色皿中，用于测定一个样本中的多种分析物（例如，用于患者档案）。我们评估了该系统的一个原型，它可以在25、30或37摄氏度下使用；在340、405和500纳米处的吸光度读数在高达2.0 A时呈线性变化。用稀释血清复溶试剂来启动反应就足够了，因为吸光度读数在复溶开始后180秒才开始。在30摄氏度下进行动力学测定的分析性能显示出良好的准确性，并且与其他方法测定肌酸激酶、淀粉酶、天冬氨酸转氨酶和γ-谷氨酰转移酶具有相关性。尿素的动力学测定以及葡萄糖、胆固醇和甘油三酯的空白校正平衡测定，对于葡萄糖给出了优异的结果，对于其他分析物给出了正确的结果。这种紧凑型分析仪将离心分析仪的分析性能与具有预先分布试剂的仪器的实用性结合在了一起。
