Horikoshi Renato J, Vertuan Hallison, de Castro Ancidériton A, Morrell Kimberly, Griffith Cara, Evans Adam, Tan Jianguo, Asiimwe Peter, Anderson Heather, José Márcia O M A, Dourado Patrick M, Berger Geraldo, Martinelli Samuel, Head Graham
Bayer Crop Science, Santa Cruz das Palmeiras, Brazil.
Plant Biotechnology, Bayer Crop Science US, Chesterfield, MO, USA.
Pest Manag Sci. 2021 Aug;77(8):3727-3736. doi: 10.1002/ps.6334. Epub 2021 Apr 1.
The pyramided genetically modified maize (Zea mays [L.]) event MON 95379, expressing the Cry1B.868 and Cry1Da_7 proteins, was designed to protect against larval feeding damage by the fall armyworm, Spodoptera frugiperda (FAW). Here, we conducted laboratory, greenhouse, and field studies to assess the dose and field efficacy of MON 95379 against FAW and inform the development of insect resistance management plans.
The Cry1B.868 and Cry1Da_7 proteins were active against susceptible FAW neonates in diet-incorporation bioassays: median lethal concentration [LC ] (95% CI) = 62.8 (42.6-87.6) μg/ml diet for Cry1B.868 and 9.4 (5.3-18.6) μg/ml diet for Cry1Da_7. In laboratory leaf disc bioassays, MON 95379 maize and experimental maize lines expressing the individual components were effective in controlling susceptible FAW. In whole-plant assays, MON 95379 controlled FAW resistant to the Cry1A.105 and Cry2Ab2 proteins. Likewise, under field conditions, MON 95379 maize expressing Cry1B.868 and Cry1Da_7 was highly effective at protecting plants against the larval feeding of FAW.
The expression of Cry1B.868 and Cry1Da_7 in MON 95379 consistently protected maize plants against larval feeding by FAW and represents an alternative to manage trait resistance issues in South America. © 2021 Bayer Crop Science-US. Pest Management Science published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.
堆叠式转基因玉米（玉米属[L.]）事件MON 95379表达Cry1B.868和Cry1Da_7蛋白，旨在抵御草地贪夜蛾（Spodoptera frugiperda，FAW）幼虫取食造成的损害。在此，我们进行了实验室、温室和田间研究，以评估MON 95379对草地贪夜蛾的剂量和田间防效，并为制定抗虫管理计划提供依据。
在饲料掺入生物测定中，Cry1B.868和Cry1Da_7蛋白对敏感草地贪夜蛾幼虫具有活性：Cry1B.868的半数致死浓度[LC]（95%置信区间）=62.8（42.6 - 87.6）μg/ml饲料，Cry1Da_7为9.4（5.3 - 18.6）μg/ml饲料。在实验室叶碟生物测定中，MON 95379玉米以及表达单个成分的实验玉米品系对控制敏感草地贪夜蛾有效。在整株植物测定中，MON 95379能控制对Cry1A.105和Cry2Ab2蛋白具有抗性的草地贪夜蛾。同样，在田间条件下，表达Cry1B.868和Cry1Da_7的MON 95379玉米在保护植株免受草地贪夜蛾幼虫取食方面非常有效。
Cry1B.868和Cry1Da_7在MON 95379中的表达持续保护玉米植株免受草地贪夜蛾幼虫取食，是南美洲管理性状抗性问题的一种替代方法。© 2021拜耳作物科学 - 美国。由John Wiley & Sons Ltd代表化学工业协会出版的《害虫管理科学》。
