State Key Laboratory of Wheat and Maize Crop Science, Henan International Laboratory for Green Pest Control, College of Plant Protection, Henan Agricultural University, Zhengzhou 450002, China.
Henan Academy of Agricultural Sciences, Zhengzhou 450002, China.
Toxins (Basel). 2022 Oct 21;14(10):721. doi: 10.3390/toxins14100721.
The fall armyworm (FAW), , causes substantial annual agricultural production losses worldwide due to its resistance to many insecticides. Therefore, new insecticides are urgently needed to more effectively control FAW. Cyclosporin A (CsA) is a secondary metabolite of fungi; little is known about its insecticidal activity, especially for the control of FAW. In this study, we demonstrate that CsA shows excellent insecticidal activity (LC = 9.69 μg/g) against FAW through significant suppression of calcineurin (CaN) activity, which is a new target for pest control. Combinations of CsA and indoxacarb, emamectin benzoate, or Vip3Aa showed independent or synergistic toxicity against FAW; however, the combination of CsA and chlorantraniliprole showed no toxicity. Sublethal doses of CsA led to decreases in FAW larval and pupal weight, pupation, emergence, mating rates, adult longevity, extended development of FAW larvae and pupae and the pre-oviposition period of adults, and increases in the proportion of pupal malformation. Importantly, CsA treatment reduced FAW ovarian size and female fecundity, which suggests that it has great potential to suppress FAW colony formation. Taken together, these results indicate that CsA has high potential as an insecticide for controlling FAW.
秋粘虫（FAW）由于对许多杀虫剂具有抗性，因此会导致全球每年的农业生产遭受重大损失。因此，迫切需要新的杀虫剂来更有效地控制 FAW。环孢菌素 A（CsA）是真菌的次生代谢物；人们对其杀虫活性知之甚少，尤其是对 FAW 的控制。在这项研究中，我们通过显著抑制钙调磷酸酶（CaN）活性来证明 CsA 对 FAW 具有出色的杀虫活性（LC = 9.69μg/g），这是害虫防治的新靶标。CsA 与茚虫威、甲维盐或 Vip3Aa 的组合对 FAW 表现出独立或增效毒性；然而，CsA 与氯虫苯甲酰胺的组合没有毒性。亚致死剂量的 CsA 导致 FAW 幼虫和蛹体重、化蛹、羽化、交配率、成虫寿命、FAW 幼虫和蛹的发育延长以及蛹畸形比例的降低。重要的是，CsA 处理降低了 FAW 的卵巢大小和雌性繁殖力，这表明它具有抑制 FAW 群体形成的巨大潜力。总之，这些结果表明 CsA 作为一种杀虫剂具有控制 FAW 的巨大潜力。
