Moehario Lucky Hartati, Tjoa Enty, Putranata Hans, Joon Shikha, Edbert Daniel, Robertus Thomas
Department of Microbiology, School of Medicine and Sciences, Atma Jaya Catholic University of Indonesia, Jakarta, Indonesia.
Institutes for Systems Genetics, Frontiers Science Center for Disease-Related Molecular Network, West China Hospital, Sichuan University, Chengdu, Sichuan, China.
J Int Med Res. 2021 Feb;49(2):300060521989893. doi: 10.1177/0300060521989893.
Automated systems are needed for the rapid and accurate diagnosis of -associated nosocomial infections among critically ill patients admitted to the intensive care unit. We assessed the performance of TDR-300B and VITEK®2 for the identification of using VITEK®-MS as the gold standard.
This analytical study employed a cross-sectional approach. First, 44 clinical isolates of were collected and refreshed. Next, a single colony of oxidase-positive, gram-negative rods (30 samples) was inoculated into a TDR-300B NF-64 card and VITEK®2 GN cassette for each isolate. Finally, bacterial identification was performed using VITEK®-MS for comparative analysis.
Compared with the results for VITEK®-MS, the congruence rates for TDR-300B and VITEK®2 were 80.76% (21/26) and 92.30% (24/26), respectively. Further, high sensitivity was observed for TDR-300B and VITEK®2 (95.45% and 100%, respectively). In addition, TDR-300B had a lower positive predictive value and accuracy than VITEK®2, albeit without significance.
Conclusively, there were no significant differences regarding the diagnostic efficiency of TDR-300B and VITEK®2 for .
需要自动化系统来快速准确地诊断重症监护病房收治的重症患者中的相关医院感染。我们以VITEK®-MS作为金标准,评估了TDR-300B和VITEK®2在鉴定方面的性能。
本分析研究采用横断面方法。首先,收集并复苏了44株临床分离株。接下来,将单个氧化酶阳性革兰氏阴性杆菌菌落(30个样本)接种到每个分离株的TDR-300B NF-64卡和VITEK®2 GN卡盒中。最后,使用VITEK®-MS进行细菌鉴定以进行比较分析。
与VITEK®-MS的结果相比,TDR-300B和VITEK®2的符合率分别为80.76%(21/26)和92.30%(24/26)。此外,观察到TDR-300B和VITEK®2具有较高的敏感性(分别为95.45%和100%)。此外,TDR-300B的阳性预测值和准确性低于VITEK®2,尽管无统计学意义。
总之,TDR-300B和VITEK®2在诊断效率方面没有显著差异。