Corrado Maria L, Knaus Tanja, Mutti Francesco G
Van't Hoff Institute for MolecularSciences, HIMS-Biocat, University of Amsterdam, Science Park 904, 1098 XH, Amsterdam, The Netherlands.
Green Chem. 2019 Dec 7;21(23):6246-6251. doi: 10.1039/C9GC03161H. Epub 2019 Oct 29.
We report an enzymatic route for the formal regio- and stereoselective aminohydroxylation of β-methylstyrene that consumes only dioxygen, ammonia and formate; carbonate is the by-product. The biocascade entails highly selective epoxidation, hydrolysis and hydrogen-borrowing alcohol amination. Thus, β-methylstyrene was converted into 1,2 and 1,2-phenylpropanolamine in 59-63% isolated yields, and up to >99.5: <0.5 dr and er.
我们报道了一种用于β-甲基苯乙烯的形式区域和立体选择性氨羟基化的酶促途径，该途径仅消耗氧气、氨和甲酸盐；副产物是碳酸盐。该生物级联反应包括高度选择性的环氧化、水解和借氢醇胺化反应。因此，β-甲基苯乙烯以59-63%的分离产率转化为1,2-和1,2-苯基丙醇胺，非对映体比例高达>99.5:<0.5，对映体过量值高达>99.5:<0.5。
