Topical Extract Enhances Wound Healing in Cutaneous Leishmaniasis.

BACKGROUND

(Tabashir) has been shown to have antimicrobial, antioxidant, and anti-inflammatory effects due to the presence of ascorbic acid, vitamin B2, flavonoid, and phenolic compounds which can be beneficial in the process of wound healing. The current study aimed to evaluate the effects of topical Tabashir extract on cutaneous leishmaniasis (CL) caused by in BALB/c mice.

METHODS

Twenty-eight female BALB/c mice (4 weeks old, 18 ± 4 grams) were injected subcutaneously in tail-base with amastigotes. Treatment started when the CL lesions were appeared and continued for 21 days. Mice were then divided into four groups: 1, treated daily with 5% of Tabashir extract gel; 2, treated daily with 10% Tabashir gel; 1, irrigated daily only with normal saline; and 2, received vehicle gel daily. The wounds' sizes were measured every 3 days, using vernier caliper. The volume densities of vessels, collagens, and hair follicles, vessels' length density, and mean diameter were soteriologically determined.

RESULTS

Tabashir enhanced wound closure rate through increasing the number of fibroblasts, collagen bundles, and vessels, according to histomorphometric evaluation while it did not affect the parasitic load. Findings of the in vitro study revealed that the extract has substantial mortality for the promastigotes.

CONCLUSION

Topical Tabashir showed promising effects on the healing process of skin wounds caused by CL in this experimental study. Further studies are suggested to find out the molecules which are involved in the healing process.