Ashkani-Esfahani Soheil, Zarifi Fariba, Asgari Qasem, Samadnejad Asal Zargari, Rafiee Shima, Noorafshan Ali
Student Research Committee, Shiraz University of Medical Sciences, Shiraz, Iran.
Department of Parasitology and Mycology, Shiraz University of Medical Sciences, Shiraz, Iran.
Adv Biomed Res. 2014 Oct 7;3:204. doi: 10.4103/2277-9175.142314. eCollection 2014.
Cutaneous Leishmaniasis is a self-limiting disease caused by protozoan parasites of the genus Leishmania, which affects the skin with full-thickness wounds, which are prone to scar formation even after treatment. Taurine (Tu) is one of the most abundant amino acids that has antioxidant and anti-inflammatory effects, which play an important role in the process of wound healing. Herein, we have investigated the effects of Tu on cutaneous Leishmaniasis wounds and L. major promastigotes.
Eighteen mice were induced with Leishmaniasis wounds (with L. Major) on the base of their tails and divided into three groups, T1: Treated with Tu injection, T2: Treated with Tu gel, and C: No treatment. Treatments were carried out every 24 hours for 21 days. The volume densities of the collagen bundles and vessels, vessel's length density and diameter, and fibroblast populations were estimated by stereological methods. Flow cytometry was used in order to investigate the direct Tu effect on parasites. The Mann-Whitney U test was used and P ≤ 0.05 was considered to be statistically significant.
The numerical density of the fibroblasts, volume density of the collagen bundles, and length densities of the vessels in groups T1 and T2 were significantly higher than in group C (P < 0.05). The fibroblast numerical density of group T1 was higher than that of group T2 (P = 0.02). Incidentally, Tu had no direct effect on L. major parasites according to the flow cytometry analysis.
Tu showed the ability to improve the wound healing process and tissue regeneration although it had no direct anti-leishmaniasis effect.
皮肤利什曼病是一种由利什曼原虫属原生动物寄生虫引起的自限性疾病,会导致皮肤出现全层伤口,即使经过治疗也容易形成疤痕。牛磺酸(Tu)是最丰富的氨基酸之一,具有抗氧化和抗炎作用,在伤口愈合过程中发挥重要作用。在此,我们研究了Tu对皮肤利什曼病伤口和硕大利什曼原虫前鞭毛体的影响。
18只小鼠尾巴基部诱导形成利什曼病伤口(感染硕大利什曼原虫),分为三组,T1组:注射Tu治疗;T2组:用Tu凝胶治疗;C组:不治疗。每24小时进行一次治疗,持续21天。采用体视学方法估计胶原束和血管的体积密度、血管的长度密度和直径以及成纤维细胞数量。使用流式细胞术研究Tu对寄生虫的直接作用。采用曼-惠特尼U检验,P≤0.05被认为具有统计学意义。
T1组和T2组成纤维细胞的数量密度、胶原束的体积密度和血管的长度密度均显著高于C组(P<0.05)。T1组成纤维细胞数量密度高于T2组(P=0.02)。顺便提一下,根据流式细胞术分析,Tu对硕大利什曼原虫没有直接作用。
Tu显示出改善伤口愈合过程和组织再生的能力,尽管它没有直接的抗利什曼病作用。