Tzartos S J, Kokla A, Walgrave S L, Conti-Tronconi B M
Hellenic Pasteur Institute, Athens, Greece.
Proc Natl Acad Sci U S A. 1988 May;85(9):2899-903. doi: 10.1073/pnas.85.9.2899.
The majority of antibodies to the acetylcholine receptor (AcChoR), both in the human disease myasthenia gravis and in its experimental models, are directed against an extracellular area of the AcChoR alpha subunit called the main immunogenic region (MIR). We have studied the binding of anti-AcChoR monoclonal antibodies (mAbs) to 26 synthetic peptides corresponding to the hydrophilic parts of the human AcChoR alpha subunit. The binding sites for eight anti-MIR mAbs and for eight anti-alpha-subunit, non-anti-MIR mAbs were localized. Anti-MIR mAbs bound to one peptide corresponding to residues 63-80 of the human alpha subunit. A second panel of peptides corresponding to the various parts of the alpha-subunit segment 63-80 was synthesized. Anti-MIR antibodies bound to a peptide that contained the alpha-subunit sequence 67-76. Thus, a main constituent loop of the MIR is localized between residues 67 and 76 of the alpha subunit.
在人类疾病重症肌无力及其实验模型中，大多数针对乙酰胆碱受体（AcChoR）的抗体都针对AcChoRα亚基的一个称为主要免疫原区（MIR）的细胞外区域。我们研究了抗AcChoR单克隆抗体（mAb）与26种对应于人AcChoRα亚基亲水部分的合成肽的结合情况。确定了8种抗MIR mAb和8种抗α亚基、非抗MIR mAb的结合位点。抗MIR mAb与一种对应于人α亚基63 - 80位残基的肽结合。合成了第二组对应于α亚基63 - 80片段不同部分的肽。抗MIR抗体与一种包含α亚基序列67 - 76的肽结合。因此，MIR的一个主要组成环位于α亚基的67和76位残基之间。
