人脑单个神经元中神经肽Y基因表达与肽储存的同步展示。
Simultaneous demonstrations of neuropeptide Y gene expression and peptide storage in single neurons of the human brain.
作者信息
Chan-Palay V, Yasargil G, Hamid Q, Polak J M, Palay S L
机构信息
Department of Neurology, University of Hospital, Zürich, Switzerland.
出版信息
Proc Natl Acad Sci U S A. 1988 May;85(9):3213-5. doi: 10.1073/pnas.85.9.3213.
A combination of in situ hybridization for neuropeptide Y mRNA that used a 32P-labeled complementary RNA probe and immunocytochemistry with polyclonal antibodies against neuropeptide Y were applied to human cortical brain samples to simultaneously localize neuropeptide Y and its mRNA. These two techniques allowed simultaneous identification of neuropeptide Y gene expression and peptide storage in single neurons of the human brain.
采用32P标记的互补RNA探针进行神经肽Y mRNA原位杂交，并结合针对神经肽Y的多克隆抗体进行免疫细胞化学，将这两种方法应用于人类大脑皮质样本，以同时定位神经肽Y及其mRNA。这两种技术能够同时鉴定人类大脑单个神经元中神经肽Y基因的表达和肽的储存情况。
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