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通过下一代测序获得的福建竹叶蛙(无尾目:蛙科)的完整线粒体DNA

The complete mitochondrial DNA for the Fujian Bamboo-leaf Frog (Anura: Ranidae) by next-generation sequencing.

作者信息

Zhong Jun-Jie, Ma Li, Lin Zhi-Hua, Ding Guo-Hua, Luo Si-Te

机构信息

Laboratory of Amphibian Diversity Investigation, College of Ecology, Lishui University, Zhejiang, China.

School of Life Sciences, Xiamen University, Xiamen, Fujian, China.

出版信息

Mitochondrial DNA B Resour. 2021 Feb 12;6(2):570-571. doi: 10.1080/23802359.2021.1875906.

DOI:10.1080/23802359.2021.1875906
PMID:33628933
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7889110/
Abstract

The complete mitochondrial DNA (mtDNA) for Li, Ye and Fei 2001 (Anura: Ranidae) was determined in this study. The length of complete mtDNA was 17,122 bp, including 13 PCGs (COI-III, ND1-6, ND4L, ATP6, ATP8 and CYTB), 25 tRNA genes, 2 rRNA genes, 2 non-coding regions of a L-strand replication origin and a control region. The overall base composition of the sequence is 28.27% T, 28.27% C, 28.52% A, and 14.94% G, with a total A + T content of 56.79%. The phylogenetic tree showed that was the sister species of , and formed a monophyletic clade with other species. These data provide a powerful tool for evolutionary biology and population genetics of genus .

摘要

本研究测定了2001年Li、Ye和Fei(无尾目:蛙科)的完整线粒体DNA(mtDNA)。完整mtDNA的长度为17,122 bp,包括13个蛋白质编码基因(COI-III、ND1-6、ND4L、ATP6、ATP8和CYTB)、25个tRNA基因、2个rRNA基因、2个L链复制起点的非编码区和1个控制区。该序列的总体碱基组成为28.27%的T、28.27%的C、28.52%的A和14.94%的G,A+T总含量为56.79%。系统发育树表明,[此处原文缺失相关物种名称]是[此处原文缺失相关物种名称]的姐妹物种,并与其他[此处原文缺失相关物种名称]物种形成一个单系类群。这些数据为[此处原文缺失相关属名]属的进化生物学和群体遗传学提供了一个有力的工具。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3bf7/7889110/82746e055c20/TMDN_A_1875906_F0001_C.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3bf7/7889110/82746e055c20/TMDN_A_1875906_F0001_C.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3bf7/7889110/82746e055c20/TMDN_A_1875906_F0001_C.jpg

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本文引用的文献

1
Sequence and analysis of the complete mitochondrial genome of the Wuchuan Odorous Frog (Anura: Ranidae).
Mitochondrial DNA B Resour. 2016 Nov 22;1(1):757-758. doi: 10.1080/23802359.2016.1219624.
2
The complete mitochondrial genome of the (Anura, Ranidae).(无尾目,蛙科)的完整线粒体基因组。
Mitochondrial DNA B Resour. 2016 Mar 28;1(1):162-163. doi: 10.1080/23802359.2016.1144112.
3
The complete mitochondrial genome of large odorous frog, (Amphibia: Ranidae) and phylogenetic analysis.大蹼铃蟾(两栖纲:蛙科)线粒体全基因组及系统发育分析
Mitochondrial DNA B Resour. 2020 Aug 9;5(3):3139-3140. doi: 10.1080/23802359.2019.1674716.
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Nucleic Acids Res. 2017 Feb 28;45(4):e18. doi: 10.1093/nar/gkw955.
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