Nasiri Samira, Shams Ghahfarokhi Masoomeh, Razzaghi Abyaneh Mehdi
Department of Mycology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran.
Department of Mycology, Pasteur Institute of Iran, Tehran, Iran.
Curr Med Mycol. 2020 Jun;6(2):30-36. doi: 10.18502/CMM.6.2.3628.
The present study was conducted to investigate the inhibitory effects of essential oil (EO) against gene expression in relation to fungal growth and some important virulence factors in .
The minimum inhibitory concentration (MIC) of EO against was determined by the Clinical and Laboratory Standards Institute M27-A4 method at a concentration range of 20-1280 μg/ml. Furthermore, the expression of gene was studied at the 0.5× MIC concentration of EO using real-time polymerase chain reaction. The proteinase and phospholipase activities, cell surface hydrophobicity (CSH), and cell membrane ergosterol (CME) content of were also assessed at the 0.5× MIC concentration of the plant EO using the approved methods. In addition, fluconazole (FLC) was used as a control antifungal drug.
The results indicated that the MIC and minimum fungicidal concentration of EO for growth were 320 and 640 μg/ml, respectively. The expression of fungal at an mRNA level and ergosterol content of yeast cells were significantly decreased by both EO (640 μg/ml) and FLC (2 μg/ml). The proteinase and phospholipase activities were also reduced in EO by 49.82% and 53.26%, respectively, while they were inhibited in FLC-treated cultures by 27.72% and 34.67%, respectively. Furthermore, the CSH was inhibited in EO- and FLC-treated cultures by 12.75% and 20.80%, respectively.
Our findings revealed that EO can be considered a potential natural compound in the development of an efficient antifungal agent against .
本研究旨在探讨香精油（EO）对[真菌名称]生长相关基因表达及一些重要毒力因子的抑制作用。
采用美国临床和实验室标准协会M27 - A4方法，在20 - 1280μg/ml浓度范围内测定EO对[真菌名称]的最低抑菌浓度（MIC）。此外，使用实时聚合酶链反应研究在EO的0.5×MIC浓度下[真菌名称]基因的表达。还采用认可的方法在植物EO的0.5×MIC浓度下评估[真菌名称]的蛋白酶和磷脂酶活性、细胞表面疏水性（CSH）以及细胞膜麦角固醇（CME）含量。另外，氟康唑（FLC）用作对照抗真菌药物。
结果表明，EO对[真菌名称]生长的MIC和最低杀菌浓度分别为320μg/ml和640μg/ml。EO（640μg/ml）和FLC（2μg/ml）均显著降低了酵母细胞mRNA水平上真菌[基因名称]的表达以及麦角固醇含量。EO还分别使蛋白酶和磷脂酶活性降低了49.82%和53.26%，而在FLC处理的培养物中分别抑制了27.72%和34.67%。此外，EO和FLC处理的培养物中CSH分别被抑制了12.75%和20.80%。
我们的研究结果表明，EO可被视为开发针对[真菌名称]的高效抗真菌剂的潜在天然化合物。
