Laboratory of Herbal Pesticides, Centre of Advanced Study in Botany, Banaras Hindu University, Varanasi, India.
Nat Prod Res. 2022 Sep;36(17):4569-4574. doi: 10.1080/14786419.2021.1994566. Epub 2021 Oct 21.
This study aimed to investigate the efficiency of chemically characterised essential oil (CcEO) against aflatoxin B (AFB) producing strain of (AF-LHP-WS-4) causing deterioration of herbal raw materials (HRM). GC-MS analysis of the EO revealed the presence of carvone (69.85%) as a dominant component. CcEO caused complete suppression of . growth and AFB secretion at 0.7 and 0.6 µL/mL, respectively. The investigation on antifungal mode of action showed that CcEO inhibited fungal growth via abrogating ergosterol biosynthesis and triggered efflux of vital cellular ions. The inhibition of AFB biosynthesis was attributed to the inhibition of cellular methylglyoxal (MG) biosynthesis. In addition, CcEO showed remarkable antioxidant activity (IC = 10.564 µL/mL) against DPPH (2,2-diphenyl-1-picrylhydrazyl) radicals. Based on overall results, it can be concluded that the CcEO may be recommended as potential antifungal agent for protection of HRM from fungal infestation and AFB contamination.
本研究旨在探讨化学特征明确的精油（CcEO）对导致草药原料（HRM）变质的黄曲霉毒素 B（AFB）产生菌株（AF-LHP-WS-4）的效率。EO 的 GC-MS 分析表明，存在香芹酮（69.85%）作为主要成分。CcEO 在 0.7 和 0.6 µL/mL 时分别完全抑制 和 AFB 的生长和分泌。对抑菌作用机制的研究表明，CcEO 通过破坏麦角固醇生物合成抑制真菌生长，并引发重要细胞离子的外排。AFB 生物合成的抑制归因于细胞甲基乙二醛（MG）生物合成的抑制。此外，CcEO 对 DPPH（2,2-二苯基-1-苦基肼）自由基表现出显著的抗氧化活性（IC = 10.564 µL/mL）。基于总体结果，可以得出结论，CcEO 可以作为保护 HRM 免受真菌侵染和 AFB 污染的潜在抗真菌剂。
