Marine College, Shandong University, Weihai, 264209, Shandong, People's Republic of China.
State Key Laboratory of Microbial Technology, Shandong University, Qingdao, 266237, Shandong, People's Republic of China.
Arch Microbiol. 2021 Jul;203(5):2229-2236. doi: 10.1007/s00203-021-02208-7. Epub 2021 Feb 25.
A novel bright yellow pigmented, Gram-stain-negative, gliding, aerobic and rod-shaped marine bacterium, designated strain S7007, was isolated from a marine sediment sample taken from Jingzi Wharf, Weihai, China. The bacterium was able to grow at 4-33 °C (optimum 28 °C), at pH 6.5-9.0 (optimum 7.0) and with 2.0-4.0% (w/v) NaCl (optimum 3.0%). According to the phylogenetic analysis based on the 16S rRNA gene sequences, strain S7007 was associated with the genus Tenacibaculum and showed highest similarity to Tenacibaculum adriaticum JCM 14633 (98.0%). The average nucleotide identity (ANI) scores of strain S7007 with T. adriaticum JCM 14633 and T. maritimum NBRC 110778 were 78.3% and 77.1%, respectively and the Genome-to-Genome Distance Calculator (dDDH) scores were 20.5% and 19.9%, respectively. The sole isoprenoid quinone was MK-6 and the major cellular fatty acids (> 10.0%) were iso-C, iso-C 3-OH, iso-C G and summed feature 3 (comprising C ω7c and/or C ω6c). The major polar lipids of strain S7007 were phosphatidylethanolamine, phosphatidyldimethylethanolamine, one unidentified lipid and two unidentified aminolipids. The genomic DNA G + C content was 30.9 mol %. The combined phenotypic data and phylogenetic inference that strain S7007 should be classified as a novel species in the genus Tenacibaculum, for which the name Tenacibaculum pelagium sp. nov. is proposed. The type strain is S7007 (= MCCC 1H00428 = KCTC 72941).
一株新型亮黄色、革兰氏阴性、滑行、需氧、杆状海洋细菌，被命名为 S7007 菌株，从中国威海荆子码头的海洋沉积物样本中分离得到。该细菌能够在 4-33°C（最适 28°C）、pH 值 6.5-9.0（最适 7.0）和 2.0-4.0%（w/v）NaCl（最适 3.0%）的条件下生长。根据 16S rRNA 基因序列的系统发育分析，菌株 S7007 与 Tenacibaculum 属密切相关，与 Tenacibaculum adriaticum JCM 14633 的相似度最高（98.0%）。与 T. adriaticum JCM 14633 和 T. maritimum NBRC 110778 的平均核苷酸同一性（ANI）评分分别为 78.3%和 77.1%，基因组到基因组距离计算器（dDDH）评分分别为 20.5%和 19.9%。唯一的异戊烯醌是 MK-6，主要的细胞脂肪酸（>10.0%）是 iso-C、iso-C 3-OH、iso-C G 和总和特征 3（包含 C ω7c 和/或 C ω6c）。菌株 S7007 的主要极性脂质是磷脂酰乙醇胺、磷脂酰二甲乙醇胺、一种未鉴定的脂质和两种未鉴定的氨基脂质。基因组 DNA G+C 含量为 30.9 mol%。综合表型数据和系统发育推断，菌株 S7007 应归类为 Tenacibaculum 属的一个新种，命名为 Tenacibaculum pelagium sp. nov.。模式菌株为 S7007（=MCCC 1H00428=KCTC 72941）。
