Fang Dong-Bo, Han Ji-Ru, Liu Yan, Du Zong-Jun
College of Marine Science, Shandong University, Weihai 264209, PR China.
State Key Laboratory of Microbial Technology, Shandong University, Jinan 250100, PR China.
Int J Syst Evol Microbiol. 2017 Nov;67(11):4857-4861. doi: 10.1099/ijsem.0.002396. Epub 2017 Oct 6.
A Gram-stain-negative, facultatively anaerobic, rod-shaped, non-flagellated and non-gliding bacterium, designated strain B011, was isolated from marine algae Gracilaria blodgettii collected from the coast of Lingshui county, Hainan, China. Phylogenetic analysis of 16S rRNA gene sequences revealed that strain B011, Seonamhaeicola algicola (96.8 % 16S rRNA gene sequence similarity) and Seonamhaeicola aphaedonensis (95.0 %) belonged to the same clade. Optimal growth occurred in the presence of 3.0 % (w/v) NaCl, at pH 6.5-7.5 and at 28 °C. Menaquinone-6 (MK-6) was the predominant respiratory quinone. The major fatty acids (>10 %) were iso-C15 : 1 G (23.4 %) and iso-C15 : 0 (22.8 %). The major polar lipids were phosphatidylethanolamine, one aminolipid and three unknown lipids. The DNA G+C content of strain B011 was 33.9 mol%. On the basis of polyphasic analysis, strain B011 is considered to represent a novel species in the genus Seonamhaeicola, for which the name Seonamhaeicolamarinus sp. nov. is proposed. The type strain is B011 (=MCCC 1H00146=NBRC 112333).
从中国海南陵水县海岸采集的江蓠藻中分离出一株革兰氏染色阴性、兼性厌氧、杆状、无鞭毛且不滑动的细菌，命名为菌株B011。16S rRNA基因序列的系统发育分析表明，菌株B011、海栖南海杆菌（Seonamhaeicola algicola，16S rRNA基因序列相似度为96.8%）和阿氏南海杆菌（Seonamhaeicola aphaedonensis，95.0%）属于同一进化枝。在3.0%（w/v）NaCl、pH 6.5 - 7.5和28 °C条件下生长最佳。甲基萘醌-6（MK-6）是主要的呼吸醌。主要脂肪酸（>10%）为异-C15 : 1 G（23.4%）和异-C15 : 0（22.8%）。主要极性脂为磷脂酰乙醇胺、一种氨基脂和三种未知脂。菌株B011的DNA G+C含量为33.9 mol%。基于多相分析，菌株B011被认为代表南海杆菌属中的一个新物种，为此提出新名称海栖南海海杆菌（Seonamhaeicola marinus sp. nov.）。模式菌株为B011（=MCCC 1H00146=NBRC 112333）。
