Zhejiang Academy of Forestry, Hangzhou, 310023, China.
Nanjing Forestry University, Nanjing, 210037, China.
Mol Biol Rep. 2021 Mar;48(3):2201-2208. doi: 10.1007/s11033-021-06228-w. Epub 2021 Feb 24.
High-throughput sequencing of the Phoebe bournei transcriptome was performed, and novel SSR markers were identified. A total of 73,518 nonredundant unigenes were assembled and annotated by sequence similarity searching in diverse public databases. A total of 40,853 SSRs were identified from 73,518 unigenes. Twenty-three pairs of polymorphic EST-SSR markers were selected from 98 markers and used for genetic analyses in 75 individuals from three P. bournei populations. The 23 pairs of markers could detect abundant genetic information from the samples (PIC = 0.769), and cross-species amplification was successfully performed in other related species. Three populations had high level of genetic diversity (He = 0.658 in average), of which the population YS from Jiangxi province had the most abundant genetic diversity (He = 0.722). The results of genetic structure analyses showed that the population YS from Jiangxi province had obvious genetic differences from the other two populations, and the genetic information of the population SX from Fujian province was related to that of the population LC from Guangdong province and the population YS. The transcriptomic resources and EST-SSR markers are valuable tools not only for the ecological conservation of P. bournei but also for phylogenetic studies.
对伯乐树转录组进行高通量测序，鉴定出新的 SSR 标记。通过在不同的公共数据库中进行序列相似性搜索，共组装和注释了 73518 个非冗余的 unigenes。从 73518 个 unigenes 中鉴定出 40853 个 SSR。从 98 个标记中选择了 23 对多态性 EST-SSR 标记，并用于来自三个伯乐树种群的 75 个个体的遗传分析。这 23 对标记可以从样本中检测到丰富的遗传信息（PIC=0.769），并且在其他相关物种中成功进行了跨物种扩增。三个种群具有高水平的遗传多样性（平均 He=0.658），其中来自江西的 YS 种群具有最丰富的遗传多样性（He=0.722）。遗传结构分析的结果表明，来自江西的 YS 种群与其他两个种群存在明显的遗传差异，来自福建的 SX 种群的遗传信息与来自广东的 LC 种群和来自江西的 YS 种群有关。转录组资源和 EST-SSR 标记不仅是伯乐树生态保护的有价值的工具，也是系统发育研究的有价值的工具。
