Chen Dan-Dan, Dong Ying, Yang Xiao-Dan
Department of Cerebrovascular Diseases, First Teaching Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300300.
Department of Radiation Ⅲ, Tumor Hospital of Jilin Province, Changchun 130012.
Zhongguo Ying Yong Sheng Li Xue Za Zhi. 2020 Sep;36(5):438-443. doi: 10.12047/j.cjap.5991.2020.093.
To investigate the effects of fatty acid binding protein 5(FABP5)- peroxisome proliferator-activated receptor gamma(PPARγ) signaling pathway on learning-memory ability and lipid metabolism in rats with vascular dementia(VD) and its mechanisms. ①VD model rats were established by ligating bilateral common carotid artery. These rats were divided into three groups: normal group (WT group), sham-operated group (sham group) and VD model group; ②WT group and FABP5 inhibitor group were set up. After four weeks, Morris water maze test was used to detect spatial learning and memory ability in rats. RT-qPCR and Western blot methods were used to detect the expressions of FABP5, PPARγ, p-PPARγ and lipoprotein lipase (LPL) in the brain at the mRNA and protein levels. The levels of TC, TG and FFA in the brain were detected by assay kits. Compared with the WT group and sham group, the learning-memory ability of the VD model and the FABP5 inhibitor group were significantly decreased, and the expressions of FABP5, PPARγ, p-PPARγ and LPL were significantly decreased at mRNA level and protein level in the brain; and the levels of TC, TG and FFA were increased significantly in the brain. FABP5 can affect the learning-memory ability and lipid metabolism in VD rats through PPARγ and LPL.
探讨脂肪酸结合蛋白5（FABP5）-过氧化物酶体增殖物激活受体γ（PPARγ）信号通路对血管性痴呆（VD）大鼠学习记忆能力和脂质代谢的影响及其机制。①通过结扎双侧颈总动脉建立VD模型大鼠。将这些大鼠分为三组：正常组（WT组）、假手术组（假手术组）和VD模型组；②设立WT组和FABP5抑制剂组。四周后，采用Morris水迷宫试验检测大鼠的空间学习和记忆能力。采用RT-qPCR和Western blot方法检测大脑中FABP5、PPARγ、p-PPARγ和脂蛋白脂肪酶（LPL）在mRNA和蛋白水平的表达。采用试剂盒检测大脑中TC、TG和FFA的水平。与WT组和假手术组相比，VD模型组和FABP5抑制剂组的学习记忆能力显著降低，大脑中FABP5、PPARγ、p-PPARγ和LPL在mRNA水平和蛋白水平的表达均显著降低；大脑中TC、TG和FFA水平显著升高。FABP5可通过PPARγ和LPL影响VD大鼠的学习记忆能力和脂质代谢。
