Gao Yin-Lai, Tian Hao-Mei, Chen Chu-Tao, Chen Xin-Yi, He Hao-Long, Zheng Hui-E, Zhu Yu, Tan Tao
College of Acupuncture-Moxibustion and Tuina, Hunan University of CM, Changsha 410208, China.
Department of Acupuncture and Moxibustion, Second Affiliated Hospital of Hunan University of CM.
Zhongguo Zhen Jiu. 2020 Oct 12;40(10):1108-12. doi: 10.13703/j.0255-2930.20190821-0008.
To observe the effect of acupuncture technique of on learning-memory ability and expressions of hippocampal vascular endothelial growth factor (VEGF) and angiogenin-1 (Ang-1) in rats with vascular dementia (VD), and to explore the mechanism of acupuncture technique of for VD.
A total of 24 male SD rats were randomly divided into a sham operation group, a model group, a medication group and an acupuncture group after Morris water maze test, 6 rats in each group. VD model was established by permanent ligation of bilateral common carotid arteries in the model group, the medication group and the acupuncture group. Treatment was given on the next day after successful modeling. The rats in the acupuncture group were treated with acupuncture at "Baihui" (GV 20), "Shenting" (GV 24), "Shuigou" (GV 26), "Dazhui" (GV 14), "Fengfu" (GV 16), "Mingmen" (GV 4), "Neiguan" (PC 6), "Daling" (PC 7) and "Laogong" (PC 8) for 30 min; the rats in the medication group were treated with nimodipine solution (0.0625 g/kg) by gavage, once a day, for 2 weeks. Morris water maze test was used to detect the behavior of rats before modeling, 2 weeks after modeling and after intervention; after intervention, the expressions of VEGF and Ang-1 protein in hippocampus were detected by Western blot.
Compared with the sham operation group, the average escape latency of rats in the model group was prolonged (<0.01), and the times of crossing the original platform were reduced (<0.01). Compared with the model group, the average escape latency of rats in the medication group and acupuncture group was significantly shortened (<0.01), and the times of crossing the original platform were increased (<0.01, <0.05). Compared with the sham operation group, the expressions of VEGF and Ang-1 protein in hippocampus in the model group were increased (<0.05, <0.01). Compared with the model group, the expressions of VEGF and Ang-1 protein in the hippocampus in the medication group and acupuncture group were significantly increased (<0.01, <0.05).
The acupuncture technique of can significantly improve the learning and memory ability of VD rats, and its mechanism may be related to up-regulating the expressions of VEGF and Ang-1 protein in hippocampus and inducing angiogenesis.
观察[具体针刺手法]针刺对血管性痴呆（VD）大鼠学习记忆能力及海马血管内皮生长因子（VEGF）和血管生成素-1（Ang-1）表达的影响，探讨[具体针刺手法]针刺治疗VD的作用机制。
24只雄性SD大鼠经Morris水迷宫测试后随机分为假手术组、模型组、药物组和针刺组，每组6只。模型组、药物组和针刺组采用永久性结扎双侧颈总动脉的方法建立VD模型。造模成功后次日开始治疗。针刺组大鼠针刺“百会”（GV 20）、“神庭”（GV 24）、“水沟”（GV 26）、“大椎”（GV 14）、“风府”（GV 16）、“命门”（GV 4）、“内关”（PC 6）、“大陵”（PC 7）、“劳宫”（PC 8），留针30 min；药物组大鼠灌胃给予尼莫地平溶液（0.0625 g/kg），每日1次，连续2周。采用Morris水迷宫测试检测大鼠造模前、造模后2周及干预后行为学变化；干预后采用Western blot检测海马组织中VEGF和Ang-1蛋白表达。
与假手术组比较，模型组大鼠平均逃避潜伏期延长（<0.01），穿越原平台次数减少（<0.01）。与模型组比较，药物组和针刺组大鼠平均逃避潜伏期明显缩短（<0.01），穿越原平台次数增加（<0.01，<0.05）。与假手术组比较，模型组海马组织中VEGF和Ang-1蛋白表达升高（<0.05，<0.01）。与模型组比较，药物组和针刺组海马组织中VEGF和Ang-1蛋白表达明显降低（<0.01，<0.05）。
[具体针刺手法]针刺可显著提高VD大鼠的学习记忆能力，其机制可能与上调海马组织中VEGF和Ang-1蛋白表达、诱导血管生成有关。
