Key Laboratory of Animal Breeding Reproduction and Molecular Design for Jiangsu Province, College of Animal Science and Technology, Yangzhou University, Yangzhou, Jiangsu 225009, China.
Key Laboratory of Animal Breeding Reproduction and Molecular Design for Jiangsu Province, College of Animal Science and Technology, Yangzhou University, Yangzhou, Jiangsu 225009, China.
Gene. 2021 May 15;780:145521. doi: 10.1016/j.gene.2021.145521. Epub 2021 Feb 22.
SPINDLIN1-Z (SPIN1Z), a member of the Spin/Ssty(Y-linked spermiogenesis specific transcript) protein family, participates in the early embryonic development process. Our previous RNA-seq analysis indicates that the level of Spin1z was abundantly expressed in male embryonic stem cells (ESCs) and primitive germ cells (PGCs), we speculate that Spin1z may play an important role in chicken male differentiation. Therefore, the loss- and gain-of-function experiments provide solid evidence that Spin1z is both necessary and sufficient to initiate male development in chicken. Furthermore, chromatin immunoprecipitation (ChIP) assay and the dual-luciferase assay was performed to further confirm that Spin1z contributed to chicken male differentiation by inhibiting the Tcf4 transcription. Our findings provide a novel insight into the molecular mechanism for chicken male differentiation.
SPINDLIN1-Z（SPIN1Z）是 Spin/Ssty（Y 连锁精子发生特异性转录物）蛋白家族的成员，参与早期胚胎发育过程。我们之前的 RNA-seq 分析表明，Spin1z 在雄性胚胎干细胞（ESCs）和原始生殖细胞（PGCs）中大量表达，我们推测 Spin1z 可能在鸡雄性分化中发挥重要作用。因此，缺失和获得功能实验为 Spin1z 是启动鸡雄性发育所必需且充分的提供了确凿的证据。此外，进行了染色质免疫沉淀（ChIP）实验和双荧光素酶实验，进一步证实 Spin1z 通过抑制 Tcf4 转录来促进鸡雄性分化。我们的研究结果为鸡雄性分化的分子机制提供了新的见解。
