School of Laboratory Medicine and Medical Sciences (SLMMS), College of Health Sciences, University of KwaZulu-Natal, Durban, South Africa; Department of Health Sciences, Faculty of Health and Applied Sciences, Namibia University of Science and Technology, Windhoek, Namibia.
Department of Life and Environmental Sciences, Polytechnic University of Marche, Ancona, Italy; Biomedical Research and Innovation Platform, South African Medical Research Council, Tygerberg, South Africa.
Cell Immunol. 2021 May;363:104313. doi: 10.1016/j.cellimm.2021.104313. Epub 2021 Feb 13.
Chronic inflammation in patients with type 2 diabetes (T2D) is associated with T-cell dysfunction. Using a rodent model, we evaluated changes in metabolic profiles, inflammation status and the expression of T-cell function markers following high-fat diet (HFD)-feeding. In addition, we assessed the modulatory effects of treatment with low-dose aspirin (LDA) and its combination with metformin (LDA + Met) on these parameters. Notably, HFD-feeding induced metabolic disorders and aggravated inflammation. Most importantly, it was associated with decreased expression of CD69 on T-helper cells but had no effect on the expression of programmed cell death 1 (PD-1). Treatment with LDA monotherapy had no effect on metabolic profiles. However, its combination with metformin ameliorated the levels of inflammation and up-regulated the expression of CD69 although it had no therapeutic effect on the levels of PD-1 expression. Therefore, alleviating inflammation and lowering glucose levels in T2D may be an effective strategy to improve T-cell function in these patients.
2 型糖尿病（T2D）患者的慢性炎症与 T 细胞功能障碍有关。我们使用啮齿动物模型，评估了高脂肪饮食（HFD）喂养后代谢谱、炎症状态和 T 细胞功能标志物表达的变化。此外，我们评估了低剂量阿司匹林（LDA）治疗及其与二甲双胍联合治疗（LDA+Met）对这些参数的调节作用。值得注意的是，HFD 喂养导致代谢紊乱和炎症加重。最重要的是，它与辅助性 T 细胞上 CD69 的表达降低有关，但对程序性细胞死亡蛋白 1（PD-1）的表达没有影响。LDA 单药治疗对代谢谱没有影响。然而，它与二甲双胍联合使用改善了炎症水平，并上调了 CD69 的表达，尽管它对 PD-1 表达水平没有治疗作用。因此，减轻 T2D 中的炎症和降低血糖水平可能是改善这些患者 T 细胞功能的有效策略。
