Key Laboratory of the Ministry of Education for Advanced Catalysis Materials, College of Chemistry and Life Sciences, College of Geography and Environmental Sciences, Zhejiang Normal University, Jinhua 321004, China.
Key Laboratory of Optic-Electric Sensing and Analytical Chemistry for Life Sciences, MOE, College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao 266042, China.
Bioelectrochemistry. 2021 Aug;140:107753. doi: 10.1016/j.bioelechem.2021.107753. Epub 2021 Feb 8.
For immobilization and signal amplification of the probes, it is feasible and promising by using porous nanomaterials as nanocarriers. Herein, a novel label-free electrochemical immmunosensor was efficiently designed for ultrasensitive detection of procalcitonin (PCT). The immunosensor was prepared by using porous silica-coated gold nanorods (Au NRs@m-SiO) to load electroactive dye thionine (Thi) on the electrode surface. Apart from the improved electrical conductivity, the porous feature highly increased the loading amount of Thi to boost the detection signals, while the good biocompatibility and protective microenvironment are beneficial to the largely improved stability for the target. For quantification of PCT, the developed immunosensor exhibited a good linear relationship in the antigen concentration range of 0.001-100 ng mL with an ultra-low limit of detection (LOD, 0.39 pg mL, S/N = 3). Moreover, the built platform was successfully applied to such assay in human serum samples. The research provides some valuable guidelines for clinical screening and diagnosis of other biomarkers.
为了固定和放大探针的信号，可以使用多孔纳米材料作为纳米载体，这是一种可行且有前景的方法。在此，我们设计了一种新型的无标记电化学免疫传感器，用于超灵敏检测降钙素原 (PCT)。该免疫传感器是通过在电极表面负载电活性染料噻嗪 (Thi) 来制备的，使用多孔硅包覆的金纳米棒 (Au NRs@m-SiO) 作为纳米载体。除了提高导电性外，多孔特性还极大地增加了 Thi 的负载量，从而增强了检测信号，而良好的生物相容性和保护微环境有利于目标的稳定性大大提高。对于 PCT 的定量检测，所开发的免疫传感器在抗原浓度为 0.001-100ng mL 的范围内表现出良好的线性关系，检测限 (LOD) 超低，为 0.39pg mL (S/N=3)。此外，该平台还成功地应用于人血清样本中的此类测定。该研究为临床筛选和其他生物标志物的诊断提供了一些有价值的指导。
