Division of Microbiology, ICAR-Indian Agricultural Research Institute, New Delhi 110012, India.
Center for Environemtal Sciences and Climate Resilient Agriculture, ICAR-Indian Agricultural Research Institute, New Delhi 110012, India.
Sci Total Environ. 2021 Jun 25;775:145826. doi: 10.1016/j.scitotenv.2021.145826. Epub 2021 Feb 13.
Methane (CH) emission in rice fields is greatly influenced by the type and quantity of nitrogenous fertilizer used. The net methane emission from paddy fields is also influenced by the activity of methane utilizing bacteria, which inhabit the flooded paddy ecosystem. Efficient methane utilizing and plant growth promoting bacteria Methylobacterium oryzae MNL7 and Paenibacillus polymyxa MaAL70, respectively were co-inoculated along with different nitrogenous fertilizer combinations in flooded paddy to assess their impact on cumulative methane emission and crop growth promotion. Co-inoculation significantly influenced the plant growth parameters of paddy, resulting in an increase in grain yield by 14.04, 11.08, and 12.38% in treatments receiving Urea, Di-ammonium Phosphate (DAP) + Urea, or farm yard manure (FYM), over their respective un-inoculated plots. Significant improvement in the rice grain nutrient quality in term of crude protein, Fe and Zn content was observed as a result of bacterial co-inoculation in FYM fertilized plots as compared to Urea and DAP+ Urea fertilized plots. Significantly higher cumulative methane emission of 63.39 kg ha was observed in uninoculated plots fertilized with FYM treatment as compared to Urea (33.83 kg ha) and DAP+Urea (31.66 kg ha) treatments. Bacterial co-inoculation significantly reduced the cumulative methane emission by 12.03, 11.47 and 6.92% in Urea, DAP+Urea, and FYM fertilized plots over their respective uninoculated treatments. Among the different fertilizer treatments, bacterial co-inoculation with urea application performed significantly better in reducing cumulative methane emission. These findings suggest that methane utilizing bacteria which also possess plant growth promoting trait can be explored for developing a novel biofertilizer for flooded paddies, as they can aid in managing both the overall methane emission and enhancing crop yield.
稻田中的甲烷（CH）排放受所用氮肥的类型和数量的影响很大。甲烷利用细菌的活性也会影响稻田的净甲烷排放，这些细菌栖息在淹没的稻田生态系统中。分别将高效利用甲烷和促进植物生长的细菌甲基杆菌（Methylobacterium oryzae）MNL7 和多粘类芽孢杆菌（Paenibacillus polymyxa）MaAL70 与不同的氮肥组合一起共同接种到淹水稻田中，以评估它们对累积甲烷排放和作物生长促进的影响。共同接种显著影响了水稻的植物生长参数，与各自未接种的处理相比，在接受尿素、磷酸二铵（DAP）+尿素或农家肥（FYM）的处理中，水稻的产量分别增加了 14.04%、11.08%和 12.38%。与尿素和 DAP+尿素施肥处理相比，在 FYM 施肥处理中，细菌共同接种导致水稻籽粒营养品质显著提高，粗蛋白、Fe 和 Zn 含量增加。与尿素（33.83 kg ha）和 DAP+尿素（31.66 kg ha）处理相比，在未接种 FYM 处理的处理中，观察到 63.39 kg ha 的累积甲烷排放量显著较高。细菌共同接种可使尿素、DAP+尿素和 FYM 施肥处理的累积甲烷排放量分别减少 12.03%、11.47%和 6.92%，与各自未接种的处理相比。在不同的肥料处理中，与尿素一起接种细菌的效果在减少累积甲烷排放方面表现更好。这些发现表明，具有植物生长促进特性的甲烷利用细菌可以被探索用于开发新型生物肥料，用于淹水稻田，因为它们可以帮助管理整体甲烷排放和提高作物产量。
