Department of Chemical Engineering and Biotechnology, Cambridge University, Cambridge, UK.
European Bioinformatics Institute, Wellcome Genome Campus, Cambridge, CB10 1SD, UK.
Sci Rep. 2021 Feb 25;11(1):4551. doi: 10.1038/s41598-021-83454-z.
We present a tomographic reconstruction algorithm (flOPT), which is applied to Optical Projection Tomography (OPT) images, that is robust to mechanical jitter and systematic angular and spatial drift. OPT relies on precise mechanical rotation and is less mechanically stable than large-scale computer tomography (CT) scanning systems, leading to reconstruction artefacts. The algorithm uses multiple (5+) tracked fiducial beads to recover the sample pose and the image rays are then back-projected at each orientation. The quality of the image reconstruction using the proposed algorithm shows an improvement when compared to the Radon transform. Moreover, when adding a systematic spatial and angular mechanical drift, the reconstruction shows a significant improvement over the Radon transform.
我们提出了一种层析重建算法（flOPT），该算法应用于光学投影层析成像（OPT）图像，对机械抖动和系统角度及空间漂移具有较强的鲁棒性。OPT 依赖于精确的机械旋转，其机械稳定性不如大型计算机断层扫描（CT）扫描系统，因此会产生重建伪影。该算法使用多个（5 个以上）跟踪基准球来恢复样本的位置，然后在每个方向上对图像射线进行反向投影。与 Radon 变换相比，使用所提出的算法进行图像重建的质量有所提高。此外，当添加系统的空间和角度机械漂移时，重建结果比 Radon 变换有显著改善。
