Sharpe James, Ahlgren Ulf, Perry Paul, Hill Bill, Ross Allyson, Hecksher-Sørensen Jacob, Baldock Richard, Davidson Duncan
Medical Research Council, Human Genetics Unit, Western General Hospital, Crewe Road, Edinburgh EH4 2XU, UK.
Science. 2002 Apr 19;296(5567):541-5. doi: 10.1126/science.1068206.
Current techniques for three-dimensional (3D) optical microscopy (deconvolution, confocal microscopy, and optical coherence tomography) generate 3D data by "optically sectioning" the specimen. This places severe constraints on the maximum thickness of a specimen that can be imaged. We have developed a microscopy technique that uses optical projection tomography (OPT) to produce high-resolution 3D images of both fluorescent and nonfluorescent biological specimens with a thickness of up to 15 millimeters. OPT microscopy allows the rapid mapping of the tissue distribution of RNA and protein expression in intact embryos or organ systems and can therefore be instrumental in studies of developmental biology or gene function.
当前的三维（3D）光学显微镜技术（去卷积、共聚焦显微镜和光学相干断层扫描）通过对标本进行“光学切片”来生成3D数据。这对可成像标本的最大厚度施加了严格限制。我们开发了一种显微镜技术，该技术使用光学投影断层扫描（OPT）来生成厚度达15毫米的荧光和非荧光生物标本的高分辨率3D图像。OPT显微镜能够快速绘制完整胚胎或器官系统中RNA和蛋白质表达的组织分布图，因此在发育生物学或基因功能研究中可能会发挥重要作用。
