可视化丝状细菌天蓝色链霉菌中基因在时间和空间上的表达。

Visualizing gene expression in time and space in the filamentous bacterium Streptomyces coelicolor.

作者信息

Schauer A, Ranes M, Santamaria R, Guijarro J, Lawlor E, Mendez C, Chater K, Losick R

机构信息

Department of Cellular and Developmental Biology, Harvard University, Cambridge, MA 02138.

出版信息

Science. 1988 May 6;240(4853):768-72. doi: 10.1126/science.3363358.

DOI:10.1126/science.3363358

PMID:3363358

Abstract

Streptomycetes are prokaryotic microorganisms that exhibit a complex, mycelial fungus-like cycle of morphological differentiation. Development involves at least two spatially separated types of cells: the branching hyphae of the substrate mycelium, which penetrate the stratum upon which the colony feeds, and the upwardly protruding hyphae of the aerial mycelium, which undergo metamorphosis into spores. The luciferase-encoding luxA and luxB operon of the luminescent marine bacterium Vibrio harveyi was used as a promoter probe to visualize gene expression in differentiating colonies of Streptomyces coelicolor. Promoters for developmental genes of several kinds gave distinctive temporal and spatial patterns of light emission.

摘要

链霉菌是原核微生物，呈现出复杂的、类似丝状真菌的形态分化周期。其发育过程至少涉及两种在空间上分离的细胞类型：基内菌丝的分支菌丝，它穿透菌落赖以生长的基质层；以及气生菌丝向上突出的菌丝，它会变态形成孢子。发光海洋细菌哈氏弧菌的编码荧光素酶的luxA和luxB操纵子被用作启动子探针，以可视化天蓝色链霉菌分化菌落中的基因表达。几种发育基因的启动子呈现出独特的发光时间和空间模式。