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固定表达发光杆菌属发光基因的大肠杆菌。

Immobilization of Escherichia coli expressing the lux genes of Xenorhabdus luminescens.

作者信息

Marincs F, White D W

机构信息

Plant Molecular Genetics Laboratory, Grasslands Research Centre, Palmerston North, New Zealand.

出版信息

Appl Environ Microbiol. 1994 Oct;60(10):3862-3. doi: 10.1128/aem.60.10.3862-3863.1994.

DOI:10.1128/aem.60.10.3862-3863.1994
PMID:7986053
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC201896/
Abstract

The luxCDABE operon of Xenorhabdus luminescens was cloned into pUC18 to make pLITE27. Expression of the lux genes from the lac promoter resulted in strong constitutive light emission by Escherichia coli DH5 carrying the recombinant lux plasmid, pLITE27. When strain DH5(pLITE27) was immobilized with sodium alginate-CaCl2, the embedded cells retained their luminescence up to 2 weeks under appropriate storage conditions.

摘要

将发光杆菌（Xenorhabdus luminescens）的luxCDABE操纵子克隆到pUC18中，构建成pLITE27。lux基因由lac启动子表达，使得携带重组lux质粒pLITE27的大肠杆菌DH5产生强烈的组成型发光。当用海藻酸钠 - 氯化钙固定DH5（pLITE27）菌株时，在适当的储存条件下，包埋的细胞在长达2周的时间内都能保持发光。

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