固定表达发光杆菌属发光基因的大肠杆菌。
Immobilization of Escherichia coli expressing the lux genes of Xenorhabdus luminescens.
作者信息
Marincs F, White D W
机构信息
Plant Molecular Genetics Laboratory, Grasslands Research Centre, Palmerston North, New Zealand.
出版信息
Appl Environ Microbiol. 1994 Oct;60(10):3862-3. doi: 10.1128/aem.60.10.3862-3863.1994.
Abstract
The luxCDABE operon of Xenorhabdus luminescens was cloned into pUC18 to make pLITE27. Expression of the lux genes from the lac promoter resulted in strong constitutive light emission by Escherichia coli DH5 carrying the recombinant lux plasmid, pLITE27. When strain DH5(pLITE27) was immobilized with sodium alginate-CaCl2, the embedded cells retained their luminescence up to 2 weeks under appropriate storage conditions.
摘要
将发光杆菌（Xenorhabdus luminescens）的luxCDABE操纵子克隆到pUC18中，构建成pLITE27。lux基因由lac启动子表达，使得携带重组lux质粒pLITE27的大肠杆菌DH5产生强烈的组成型发光。当用海藻酸钠 - 氯化钙固定DH5（pLITE27）菌株时，在适当的储存条件下，包埋的细胞在长达2周的时间内都能保持发光。
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1
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Science. 1990 Aug 17;249(4970):778-81. doi: 10.1126/science.249.4970.778.
2
Use of a transposon with luciferase as a reporter to identify environmentally responsive genes in a cyanobacterium.使用带有荧光素酶作为报告基因的转座子来鉴定蓝细菌中的环境响应基因。
Proc Natl Acad Sci U S A. 1991 Jun 15;88(12):5355-9. doi: 10.1073/pnas.88.12.5355.
3
Bioluminescence (lux) expression in the anaerobe Clostridium perfringens.产气荚膜梭菌厌氧菌中的生物发光（勒克斯）表达。
FEMS Microbiol Lett. 1993 Feb 1;106(3):265-70. doi: 10.1111/j.1574-6968.1993.tb05974.x.
4
The use of bacterial luciferase genes as reporter genes in Lactococcus: regulation of the Lactococcus lactis subsp. lactis lactose genes.细菌荧光素酶基因在乳酸乳球菌中作为报告基因的应用：乳酸乳球菌乳亚种乳糖基因的调控
J Gen Microbiol. 1993 Jul;139(7):1495-501. doi: 10.1099/00221287-139-7-1495.
5
Bacterial bioluminescence: organization, regulation, and application of the lux genes.细菌生物发光：lux基因的组织、调控及应用
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