Gong Hua, Wang Qing, Lai Yingtiao, Zhao Changchen, Sun Chenwen, Chen Zonghui, Tao Jiafa, Huang Zhibin
Key Lab of Aquatic Animal Immune Technology of Guangdong Province, Key Lab of Fishery Drug Development of Ministry of Agriculture and Rural Affairs, Pearl River Fisheries Research Institute of Chinese Academy of Fishery Sciences, Guangzhou, China.
Front Immunol. 2021 Feb 9;11:622387. doi: 10.3389/fimmu.2020.622387. eCollection 2020.
Immersion vaccination relies on the response of fish mucosa-associated lymphoid tissues, the Crucian carp () and Grouper () were researched in this paper to examine local mucosal immune responses and associated humoral system responses following immersion vaccination. We administered 1.5 × 10 CFU/ml formalin-inactivated cells and measured mucus and serum antibody titers as well as IgM, MHC II mRNA levels in immune organs. The mucosal antibody response preceded the serum response indicating a role for local mucosal immunity in immersion vaccination. IgM and MHC II mRNA levels were relatively greater for the spleen and head kidney indicating the importance and central position of systemic immunity. Expression levels were also high for the gills while skin levels were the lowest. IgM and MHC II mRNA levels were altered over time following vaccination and the hindgut, liver and spleen were similar indicating a close relationship, so the absolute value of is used to analyze the correlation among different organs immunized. It can be inferred the existence of an internal immune molecular mechanism for Immune synergy hindgut-liver-spleen, from the peak time (14 day), the relative ratio of genes expression in the same tissues between the immunized grouper and the control group (26 times), and Pearson correlation coefficient (0.8<||<1). Injection challenges with live indicated that the relative protection rates for the crucian carp and Grouper was basically the same at 44.4% and 47.4%, respectively. It is believe that crucian carp may be used as a substitute for the valuable grouper in immunity experiment, just from aspect of the relative percent survival (RPS) and how it changes with time. But they were not consistent about the IgM mRNA expression between that of crucian carp and grouper after immersion the vaccine.
浸泡式疫苗接种依赖于鱼类黏膜相关淋巴组织的反应，本文以鲫鱼（）和石斑鱼（）为研究对象，检测浸泡式疫苗接种后的局部黏膜免疫反应及相关体液系统反应。我们给予1.5×10 CFU/ml福尔马林灭活的 细胞，并测量黏液和血清抗体滴度以及免疫器官中IgM、MHC II mRNA水平。黏膜抗体反应先于血清反应，表明局部黏膜免疫在浸泡式疫苗接种中发挥作用。脾脏和头肾的IgM和MHC II mRNA水平相对较高，表明全身免疫的重要性和核心地位。鳃的表达水平也较高，而皮肤的水平最低。接种疫苗后，IgM和MHC II mRNA水平随时间变化，后肠、肝脏和脾脏的情况相似，表明它们之间关系密切，因此用 的绝对值来分析不同免疫器官之间的相关性。从峰值时间（14天）、免疫石斑鱼与对照组同一组织中基因表达的相对比值（26倍）以及Pearson相关系数（0.8<||<1）可以推断，存在免疫协同后肠-肝脏-脾脏的内部免疫分子机制。用活的 进行注射攻毒表明，鲫鱼和石斑鱼的相对保护率基本相同，分别为44.4%和47.4%。仅从相对存活率（RPS）及其随时间的变化来看，认为鲫鱼在免疫实验中可以替代珍贵的石斑鱼。但在浸泡 疫苗后，鲫鱼和石斑鱼的IgM mRNA表达情况并不一致。
