Guo Wenjie, Han Rui, Xu Weizhen, Lu Zijun, Li Yanwei, Dan Xueming, Mo Zequan
University Joint Laboratory of Guangdong Province, Hong Kong and Macao Region on Marine Bioresource Conservation and Exploitation, College of Marine Sciences, South China Agricultural University, Guangzhou, China.
Henry Fok School of Biology and Agriculture, Shaoguan University, Shaoguan, China.
Front Immunol. 2023 Jul 13;14:1162975. doi: 10.3389/fimmu.2023.1162975. eCollection 2023.
, which causes columnaris disease, is responsible for significant mortality in grass carp. Vaccination is a safe and effective measure to combat this disease, and this study aimed to investigate the immune protective effects of different treatments using an inactivated vaccine. The vaccine was prepared by inactivating the bacteria with 0.05% formaldehyde at 4°C for 24 hours. The experiments involving grass carp were divided into two parts. In Experiment 1, the immune effects of two isolates, JX-01 (genomovar I) and MU-04 (genomovar II), were compared, along with the impact of white oil adjuvant and the number of immunizations. The results showed that when the white oil adjuvant was used as a booster, the relative percent survival (RPS) of the JW2 group and MW2 group after 8 weeks of the first immunization was 34% and 61%, respectively. In Experiment 2, only the MU-04 (genomovar II) isolate was used as an antigen, with the white oil adjuvant as a booster. The effects of different doses (CFU=10, 10, and 10 bacteria/mL) on immune responses were compared, and the RPS values in the MW6, MW7, and MW8 groups after 4 weeks of the first immunization were found to be 38%, 57%, and 71%, respectively. Furthermore, in the cross-antigen protection experiment, the MW2 group exhibited an RPS of 55% against the JX-01 isolate, which was significantly higher than the control group (33%). These findings suggest that an inactivated vaccine comprising an appropriate antigen isolate when administered with a white oil adjuvant as a booster, can provide effective protection in grass carp.
柱状黄杆菌会引发草鱼的柱状病，导致草鱼大量死亡。疫苗接种是对抗这种疾病的一种安全有效的措施，本研究旨在调查使用灭活疫苗的不同处理方法的免疫保护效果。该疫苗是通过在4℃下用0.05%甲醛将细菌灭活24小时制备而成的。涉及草鱼的实验分为两部分。在实验1中，比较了两个菌株JX - 01（基因组I型）和MU - 04（基因组II型）的免疫效果，以及白油佐剂和免疫次数的影响。结果表明，当使用白油佐剂作为加强免疫时，首次免疫8周后JW2组和MW2组的相对存活率（RPS）分别为34%和61%。在实验2中，仅使用MU - 04（基因组II型）菌株作为抗原，以白油佐剂作为加强免疫。比较了不同剂量（CFU = 10⁶、10⁷和10⁸个细菌/毫升）对免疫反应的影响，发现首次免疫4周后MW6、MW7和MW8组的RPS值分别为38%、57%和71%。此外，在交叉抗原保护实验中，MW2组对JX - 01菌株的RPS为55%，显著高于对照组（33%）。这些研究结果表明，当使用白油佐剂作为加强免疫时，包含合适抗原菌株的灭活疫苗可以为草鱼提供有效的保护。
