Ambrosini Stefano, Sega Davide, Santi Chiara, Zamboni Anita, Varanini Zeno, Pandolfini Tiziana
Dipartimento di Biotecnologie, Università degli Studi di Verona, Verona, Italy.
Front Plant Sci. 2021 Feb 9;12:600623. doi: 10.3389/fpls.2021.600623. eCollection 2021.
Protein hydrolysates (PHs) are a class of plant biostimulants used in the agricultural practice to improve crop performance. In this study, we have assessed the capacity of a commercial PH derived from bovine collagen to mitigate drought, hypoxic, and Fe deficiency stress in . As for the drought and hypoxic stresses, hydroponically grown plants treated with the PH exhibited an increased growth and absorption area of the roots compared with those treated with inorganic nitrogen. In the case of Fe deficiency, plants supplied with the PH mixed with FeCl showed a faster recovery from deficiency compared to plants supplied with FeCl alone or with FeEDTA, resulting in higher SPAD values, a greater concentration of Fe in the leaves and modulation in the expression of genes related to Fe. Moreover, through the analysis of circular dichroism spectra, we assessed that the PH interacts with Fe in a dose-dependent manner. Various hypothesis about the mechanisms of action of the collagen-based PH as stress protectant particularly in Fe-deficiency, are discussed.
蛋白质水解物（PHs）是一类在农业实践中用于改善作物性能的植物生物刺激剂。在本研究中，我们评估了一种源自牛胶原蛋白的商业PH减轻干旱、缺氧和缺铁胁迫的能力。至于干旱和缺氧胁迫，与用无机氮处理的植物相比，用PH处理的水培植物根系生长和吸收面积增加。在缺铁的情况下，与单独供应FeCl或FeEDTA的植物相比，供应与FeCl混合的PH的植物从缺铁状态恢复得更快，导致更高的SPAD值、叶片中更高的铁浓度以及与铁相关基因表达的调节。此外，通过圆二色光谱分析，我们评估了PH与铁以剂量依赖的方式相互作用。讨论了关于基于胶原蛋白的PH作为应激保护剂，特别是在缺铁情况下的作用机制的各种假设。
