School of Chemistry and Chemical Engineering, Southeast University, Nanjing 211189, China.
J Mater Chem B. 2021 Mar 17;9(10):2462-2468. doi: 10.1039/d0tb02851g.
In this work, an NIR-activated fluorescent dye naphthalimide-thioether-cyanine (NPSCY) was developed for the photodynamic treatment of cancer cells. In this dye, naphthalimide and cyanine were selected as the two fluorophores, which were linked by the thioether group. Under 660 nm irradiation, NPSCY could produce 1O2 rapidly, suggesting the potential for photodynamic therapy. Cys can be considered as one of the markers of cancer cells and NPSCY could distinguish Cys from three channels (433 nm, 475 nm, 733 nm) due to the bilateral recognition of the thioether group, which was helpful for accurately locating cancer cells. Fortunately, NPSCY could also produce 1O2 after being reacted with the intracellular biological thiols, which also avoided the inactivation of the photosensitizer in cancer cells. The co-localization coefficient of 0.873 indicated that the cyanine group promoted the aggregation of NPSCY in mitochondria. This photosensitizer showed low dark toxicity and high phototoxicity. Meanwhile, the half-maximal inhibitory concentration (IC50) was calculated to be 3.7 μM. NPSCY could inhibit cell migration after irradiation at 660 nm.
在这项工作中，开发了一种近红外激活荧光染料萘酰亚胺硫醚菁（NPSCY），用于癌细胞的光动力治疗。在这种染料中，萘酰亚胺和菁被选为两个荧光团，通过硫醚基团连接。在 660nm 照射下，NPSCY 可以快速产生 1O2，表明其具有光动力治疗的潜力。半胱氨酸可以被认为是癌细胞的标志物之一，由于硫醚基团的双侧识别，NPSCY 可以从三个通道（433nm、475nm、733nm）区分半胱氨酸，这有助于精确定位癌细胞。幸运的是，NPSCY 也可以在与细胞内生物硫醇反应后产生 1O2，这也避免了癌细胞中光敏剂的失活。0.873 的共定位系数表明，菁基团促进了 NPSCY 在线粒体中的聚集。这种光敏剂显示出低暗毒性和高光毒性。同时，计算出的半最大抑制浓度（IC50）为 3.7μM。NPSCY 可以在 660nm 照射后抑制细胞迁移。
