Ward Leigh C
School of Chemistry and Molecular Biosciences, 1974The University of Queensland, St. Lucia, Australia.
Appl Spectrosc. 2021 Jun;75(6):698-705. doi: 10.1177/00037028211002532. Epub 2021 Mar 30.
Conventional methods for measuring the concentration of deuterium in body fluids are by either isotope ratio mass spectrometry or Fourier transform infrared transmission (FT-IR) spectroscopy. The latter method is often preferred as it is less expensive and time consuming; however, having a lower sensitivity means a larger sample volume is required. This study investigated an alternative FT-IR spectroscopic method, attenuated total reflection Fourier transform infrared spectroscopy (ATR FT-IR), which has the potential to provide shorter analysis times while requiring smaller sample volumes. Deuterium was assayed using ATR FT-IR in plasma in the concentration range 0.5 to 2.5 mg mL, typical of those observed in tracer dilution measurements of total body water. Minimal sample preparation was required and analysis time was substantially decreased compared to transmission FT-IR. Samples were analyzed with high precision (coefficient of variation (CV) < 0.5%). Precision of assay was maintained when assaying plasma volumes of only 10 µL The application of the method to the determination of total body water in humans and animals (horses) was demonstrated. A rapid and simple method for the measurement of deuterium in plasma is described that only requires very small sample volumes, rendering the method suitable for use in pediatrics where blood sampling is required to be kept to a minimum.
测量体液中氘浓度的传统方法是采用同位素比率质谱分析法或傅里叶变换红外透射（FT-IR）光谱法。后一种方法通常更受青睐，因为它成本更低且耗时更少；然而，灵敏度较低意味着需要更大的样本量。本研究调查了另一种傅里叶变换红外光谱法，即衰减全反射傅里叶变换红外光谱法（ATR FT-IR），它有可能在需要更小样本量的同时提供更短的分析时间。在血浆中使用ATR FT-IR测定氘，浓度范围为0.5至2.5mg/mL，这是在全身水的示踪剂稀释测量中观察到的典型浓度范围。与透射FT-IR相比，所需的样本制备最少，分析时间大幅缩短。样本分析具有高精度（变异系数（CV）<0.5%）。仅测定10μL血浆体积时，测定精度得以保持。该方法在测定人和动物（马）的全身水方面的应用得到了证明。本文描述了一种快速且简单的测量血浆中氘的方法，该方法仅需要非常小的样本量，使得该方法适用于需要将采血降至最低限度的儿科。
