School of Life Sciences, Beijing University of Chinese Medicine, Beijing 102488, China.
School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 102488, China.
Appl Spectrosc. 2023 Jul;77(7):774-785. doi: 10.1177/00037028231170597. Epub 2023 Apr 26.
Herbal powder preparations (HPPs) are common forms of traditional medicine made by blending the powder of two or more ingredients. The first step to ensure the safety and efficacy of HPPs is to confirm the prescribed ingredients and screen the abnormal ingredients. With the help of attenuated total reflection Fourier transform infrared spectroscopy (ATR FT-IR) imaging or mapping, the particles of different ingredients in an HPP sample can be measured individually. In this way, the overlapped absorption signals of different ingredients in the ATR FT-IR spectrum of the bulk sample can be isolated in the ATR FT-IR spectra of the microscopic particles, which leads to the substantial increase of the specificity and sensitivity of the infrared spectral identification method. The characteristic particles of each ingredient can be identified by the objective comparison of the microscopic ATR FT-IR spectra against the reference spectra based on the correlation coefficients. Since the ATR FT-IR imaging or mapping tests of HPPs are free of the separation preprocess, multiple organic and inorganic ingredients are able to be recognized by a single identification procedure simultaneously rather than by different separation and identification procedures. As an example, the ATR FT-IR mapping method was used in this research to successfully identify three prescribed ingredients and two abnormal ingredients in oral ulcer pulvis, which is a classic HPP for oral ulcer in traditional Chinese medicine. The results show the feasibility of the ATR FT-IR microspectroscopic identification method for the objective and simultaneous identification of the prescribed and abnormal ingredients of HPPs.
草药粉末制剂（HPPs）是一种常见的传统医学形式，由两种或多种成分的粉末混合而成。确保 HPPs 安全有效的第一步是确认规定的成分并筛选异常成分。借助衰减全反射傅里叶变换红外光谱（ATR FT-IR）成像或映射，可以单独测量 HPP 样品中不同成分的颗粒。通过这种方式，可以在大块样品的 ATR FT-IR 光谱中分离出微观颗粒中不同成分的重叠吸收信号，从而大大提高了红外光谱识别方法的特异性和灵敏度。通过基于相关系数的客观比较微观 ATR FT-IR 光谱与参考光谱，可以识别每个成分的特征颗粒。由于 HPP 的 ATR FT-IR 成像或映射测试无需分离预处理，因此可以通过单个识别程序同时识别多种有机和无机成分，而不是通过不同的分离和识别程序。例如，本研究采用 ATR FT-IR 映射法成功鉴定了口腔溃疡散这一经典的中药口腔溃疡 HPP 中的三种规定成分和两种异常成分。结果表明，ATR FT-IR 微光谱识别方法对于 HPP 的规定成分和异常成分的客观和同时识别是可行的。
