Pelzer H, Schwarz A, Heimburger N
Department for Blood Coagulation, Behringwerke AG, Marburg, West Germany.
Thromb Haemost. 1988 Feb 25;59(1):101-6.
An enzyme-linked immunosorbent assay (ELISA) was developed for the determination of thrombin-antithrombin III complex (TAT) in human plasma. The test system follows the sandwich principle and uses two different antibodies directed against human thrombin and human antithrombin III, respectively. The antibodies bind selectively to the corresponding antigen moieties of TAT. The assay was calibrated with definite concentrations of preformed purified TAT added to TAT-poor plasma. The lower limit of sensitivity of the assay was 0.5 microgram/l. Mean coefficients of variation of 4.2% (intraassay) and 3.5% (interassay) were found for TAT concentrations between 2 and 60 micrograms/l. A reference range from 0.85 to 3.2 micrograms/l was calculated from TAT concentration in plasma samples from 88 healthy donors (mean value +/- SD: 1.45 +/- 0.4 micrograms/l). In plasma samples from patients with pulmonary embolism (n = 17), TAT concentrations between 3 and 25 micrograms/l were measured. In 15 patients with deep vein thrombosis, TAT was found up to 3 to 25 micrograms/l. From these data we conclude that measurement of TAT can be a sensitive parameter for specific detection of a latent activation of the clotting pathway.
开发了一种酶联免疫吸附测定法（ELISA），用于测定人血浆中的凝血酶 - 抗凝血酶III复合物（TAT）。该测试系统遵循夹心原理，分别使用两种针对人凝血酶和人抗凝血酶III的不同抗体。这些抗体选择性地结合TAT的相应抗原部分。该测定法用添加到低TAT血浆中的一定浓度的预先形成的纯化TAT进行校准。该测定法的灵敏度下限为0.5微克/升。对于2至60微克/升的TAT浓度，测定法的平均变异系数为4.2%（批内）和3.5%（批间）。根据88名健康供体血浆样本中的TAT浓度计算出参考范围为0.85至3.2微克/升（平均值±标准差：1.45±0.4微克/升）。在肺栓塞患者的血浆样本（n = 17）中，测得的TAT浓度在3至25微克/升之间。在15名深静脉血栓形成患者中，发现TAT高达3至25微克/升。从这些数据我们得出结论，TAT的测量可以是凝血途径潜在激活特异性检测的一个敏感参数。
