Key Laboratory of Optoelectronic Technology & Systems (Chongqing University), Ministry of Education, Chongqing 400044, China.
J Mater Chem B. 2021 Apr 7;9(13):2998-3004. doi: 10.1039/d0tb02531c. Epub 2021 Feb 26.
Considering the limited sensitivity and accuracy of single-signal assay strategies, the multi-signal assay strategy has sparked significant excitement in recent years. In this study, for the first time, we reported a one-pot method in situ synthesis of carbon-containing nanoparticles (CNPs) via p-aminophenol (AP) and diethylenetriamine (DETA). The CNP solution exhibits yellow and light blue fluorescence under UV-light. Moreover, the CNPs exhibited excellent photoluminescence stability even under extreme conditions. Inspired by the alkaline phosphatase (ALP)-triggered specific catalytic reaction, we constructed an ultrasensitive fluorescence and colorimetric two-channel strategy for monitoring the ALP activity. By optimizing the detection parameters, the detection limits for both fluorometric and colorimetric were 0.05 mU mL. Moreover, the strategy showed high specificity and was successfully applied to monitor the ALP activity level in human serum samples. The analytical strategy opened a new window for the detection of the ALP activity, screening of the ALP inhibitor, and disease diagnosis.
考虑到单信号检测策略的灵敏度和准确性有限，多信号检测策略近年来引起了极大的关注。在这项研究中，我们首次报道了一种通过对氨基苯酚（AP）和二乙烯三胺（DETA）一锅法原位合成含碳纳米粒子（CNPs）的方法。所得的 CNPs 溶液在紫外光下呈现黄色和浅蓝色荧光。此外，即使在极端条件下，CNPs 也表现出优异的光致发光稳定性。受碱性磷酸酶（ALP）触发的特异性催化反应的启发，我们构建了一种用于监测 ALP 活性的超灵敏荧光和比色双通道策略。通过优化检测参数，荧光和比色检测的检出限均为 0.05 mU mL-1。此外，该策略具有高特异性，并成功应用于监测人血清样品中的 ALP 活性水平。该分析策略为 ALP 活性检测、ALP 抑制剂筛选和疾病诊断开辟了新的窗口。
