Department of Food and Nutrition, Sookmyung Women's University, Seoul 04310, Korea.
Food Standard Research Center, Korea Food Research Institute, Jeollabuk-do 55365, Korea.
J Food Prot. 2021 Jul 1;84(7):1141-1149. doi: 10.4315/JFP-20-437.
An investigation of the pathogenic characteristics of isolates of Vibrio parahaemolyticus was conducted by identifying the pathogenic tdh gene and then performing adherence and cytotoxicity assays. Genome sequences of the seafood isolates were analyzed using the Illumina HiSeq 2500 platform. The isolated strains were then mapped by comparing the genomes to the reference genome, and variations in the nucleotide sequences and amino acids were identified with the CLC Genomics Workbench program. The tdh gene was identified in four isolates of V. parahaemolyticus, of which three-SMFM201809-CPC7-3, SMFM201809-CF8-2, and SMFM201809-CF8-3-showed high cytotoxicity and differences in cell adhesion. These isolates were selected to identify virulence factors and genomic variations. All three isolates had the same virulence factors, such as adherence, secretion system, and toxin. In addition, amino acid variants were identified in the regions of type IV pilus, T3SS1 and T3SS1 secreted effectors, and thermolabile hemolysin. These results indicate that variations in amino acids found in regions related to adherence and cytotoxicity result in differences in adhesion efficiency and cytotoxicity; therefore, the isolates with these variations may cause more serious foodborne illness compared with other strains.
本研究通过鉴定致病性 tdh 基因，并进行黏附和细胞毒性检测，对副溶血性弧菌分离株的致病特征进行了研究。采用 Illumina HiSeq 2500 平台对海鲜分离株的基因组序列进行了分析。通过将基因组与参考基因组进行比较，对分离株进行了映射，并用 CLC Genomics Workbench 程序识别核苷酸序列和氨基酸的变异。在 4 株副溶血性弧菌分离株中鉴定出 tdh 基因，其中 3 株-SMFM201809-CPC7-3、SMFM201809-CF8-2 和 SMFM201809-CF8-3-显示出高细胞毒性和细胞黏附差异。选择这些分离株来鉴定毒力因子和基因组变异。这 3 株分离株均具有相同的毒力因子，如黏附、分泌系统和毒素。此外，还在 IV 型菌毛、T3SS1 和 T3SS1 分泌效应子以及不耐热溶血素的相关区域鉴定出氨基酸变异。这些结果表明，与黏附和细胞毒性相关区域的氨基酸变异导致黏附效率和细胞毒性的差异；因此，与其他菌株相比，具有这些变异的分离株可能会导致更严重的食源性疾病。
