Lomonosov Moscow State University, Moscow 119991, Russia.
Lomonosov Moscow State University, Moscow 119991, Russia.
Int J Biol Macromol. 2021 Apr 30;177:455-462. doi: 10.1016/j.ijbiomac.2021.02.169. Epub 2021 Feb 23.
Anionic liposomes were electrostatically adsorbed onto the surface of cationic chitosan particles cross-linked by sulfate anions, forming multi-liposomal containers (MLCs) for encapsulation and delivery of bioactive substances. An increase in molecular mass of chitosan from 30 to 300 kDa results in a size increase of chitosan particles, from 200 to 400 nm. Being saturated by liposomes, chitosan particles give MLCs of 320-540 nm. Each chitosan particle carries between 60 and 200 liposomes. The proteolytic complex Morikrase, a mixture of enzymes with various specificities, induces degradation of MLCs down to particles of size 10-15 nm; the higher the molecular mass of chitosan, the slower the enzyme-induced MLCs' degradation. pH variation within 5.5-7 and cholesterol incorporation into the liposomal membrane both have a minor effect on the rate of MLCs' biodegradation. Both the MLCs and the products of their biodegradation show low cytotoxicity. These results are of interest for constructing biodegradable capacious carriers of bioactive substances.
阴离子脂质体通过静电吸附到带正电荷的壳聚糖颗粒表面，这些颗粒由硫酸根阴离子交联，形成用于封装和递送生物活性物质的多脂质体容器（MLC）。壳聚糖的分子量从 30 增加到 300 kDa 会导致壳聚糖颗粒的尺寸从 200 增加到 400nm。被脂质体饱和后，壳聚糖颗粒形成 320-540nm 的 MLC。每个壳聚糖颗粒携带 60 到 200 个脂质体。蛋白水解复合物 Morikrase 是一种具有各种特异性的酶混合物，可诱导 MLC 降解至 10-15nm 大小的颗粒；壳聚糖的分子量越高，酶诱导的 MLC 降解速度越慢。pH 值在 5.5-7 之间变化以及胆固醇掺入脂质体膜都对 MLC 的生物降解速率有较小的影响。MLC 及其生物降解产物均表现出低细胞毒性。这些结果对于构建生物活性物质的可生物降解的大容量载体具有重要意义。
