Orthopeadic Dept. Xinqiao Hospital, Army Medical University, Chongqing, China.
Orthopeadic Dept. Xinqiao Hospital, Army Medical University, Chongqing, China.
Gene. 2021 May 25;782:145537. doi: 10.1016/j.gene.2021.145537. Epub 2021 Feb 23.
Detection of TCGA data revealed that WIPI1 is highly expressed in osteosarcoma cells. So we explore the mechanisms of WIPI1 affecting the proliferation of osteosarcoma cells through Affymetrix microarray analysis. Functional analysis of differentially expressed genes shows that the classical signaling pathways affecting tumor formation and development have changed significantly. By fitting analysis, it is speculated that the WIPI1 may function in the direction of osteosarcoma by regulating the expression of multiple cell cycle-related genes such as CDKN1A, CDK4 and CCND1. Therefore, the key genes are selected for RT-PCR and Western-blot verification. Combined with flow and other means, WIPI1 may affect the cell cycle and the osteosarcoma by regulating the expression of CDKN1A, CDK4 and CCND1. To verify the results, the effect of WIPI1 on cell proliferation was quantified by MTT, cell counts and nude mouse tumorigenicity assay. The results showed that WIPI1 promotes osteosarcoma cell proliferation.
TCGA 数据检测显示，WIPI1 在骨肉瘤细胞中高表达。因此，我们通过 Affymetrix 微阵列分析探索了 WIPI1 影响骨肉瘤细胞增殖的机制。差异表达基因的功能分析表明，影响肿瘤形成和发展的经典信号通路发生了显著变化。通过拟合分析，推测 WIPI1 可能通过调节 CDKN1A、CDK4 和 CCND1 等多个细胞周期相关基因的表达，在骨肉瘤的方向上发挥作用。因此，选择关键基因进行 RT-PCR 和 Western-blot 验证。结合流式等手段，WIPI1 可能通过调节 CDKN1A、CDK4 和 CCND1 的表达来影响细胞周期和骨肉瘤。为了验证结果，通过 MTT、细胞计数和裸鼠成瘤实验定量分析了 WIPI1 对细胞增殖的影响。结果表明，WIPI1 促进骨肉瘤细胞增殖。
