Institute of Nonferrous Metallurgy and Purest Materials, TU Bergakademie Freiberg, Leipziger Str. 32, 09599 Freiberg, Germany; Helmholtz Institute Freiberg for Resource Technology, Helmholtz-Zentrum Dresden-Rossendorf, Bautzner Landstraße 400, 01328 Dresden, Germany.
Institute of Resource Ecology, Helmholtz-Zentrum Dresden-Rossendorf, Bautzner Landstraße 400, 01328 Dresden, Germany.
J Hazard Mater. 2021 Jul 15;414:125366. doi: 10.1016/j.jhazmat.2021.125366. Epub 2021 Feb 17.
Here we provide a proof of principle for an application-oriented concept for the peptide-based recovery of gallium in industrial wastewater, which was supported by biosorption studies with a real wastewater sample. We investigated the interaction of the gallium-binding peptides TMHHAAIAHPPH, NYLPHQSSSPSR, SQALSTSRQDLR, HTQHIQSDDHLA, and NDLQRHRLTAGP with gallium and arsenic through different experimental and computational approaches. Data obtained from isothermal titration microcalorimetry indicated a competitive influence by the presence of acetate ions with an exothermic contribution to the otherwise endothermic peptide gallium interactions. For peptide HTQHIQSDDHLA, a stabilizing influence of acetate ions on the metal peptide interaction was found. Peptide NYLPHQSSSPSR showed the highest affinity for gallium in ITC studies. Computational modeling of peptide NYLPHQSSSPSR was used to determine interaction parameters and to explain a possible binding mechanism. Furthermore, the peptides were immobilized on polystyrene beads. Thus, we created a novel and exceptionally robust peptide-based material for the biosorption of gallium from an aqueous solution. Data obtained from isothermal titration microcalorimetry indicated a competitive influence by the presence of acetate ions with an exothermic contribution to the otherwise endothermic peptide gallium interactions. For peptide HTQHIQSDDHLA, a stabilizing influence of acetate ions on the metal peptide interaction was found. Peptide NYLPHQSSSPSR showed the highest affinity for gallium in ITC studies. Computational modeling of peptide NYLPHQSSSPSR was used to determine interaction parameters and to explain a possible binding mechanism. Furthermore, the peptides were immobilized on polystyrene beads. Thus, we created a novel and exceptionally robust peptide-based material for the biosorption of gallium from an aqueous solution.
在这里，我们提供了一个基于肽的从工业废水中回收镓的应用导向概念的原理证明，该概念得到了真实废水样品的生物吸附研究的支持。我们通过不同的实验和计算方法研究了结合肽 TMHHAAIAHPPH、NYLPHQSSSPSR、SQALSTSRQDLR、HTQHIQSDDHLA 和 NDLQRHRLTAGP 与镓和砷的相互作用。等温滴定量热法获得的数据表明，醋酸根离子的存在具有竞争影响，对原本吸热的肽-镓相互作用有放热贡献。对于肽 HTQHIQSDDHLA，发现醋酸根离子对金属-肽相互作用有稳定影响。在 ITC 研究中，肽 NYLPHQSSSPSR 对镓表现出最高的亲和力。肽 NYLPHQSSSPSR 的计算建模用于确定相互作用参数并解释可能的结合机制。此外，肽被固定在聚苯乙烯珠上。因此，我们创建了一种新型的、异常坚固的基于肽的材料，用于从水溶液中吸附镓。等温滴定量热法获得的数据表明，醋酸根离子的存在具有竞争影响，对原本吸热的肽-镓相互作用有放热贡献。对于肽 HTQHIQSDDHLA，发现醋酸根离子对金属-肽相互作用有稳定影响。在 ITC 研究中，肽 NYLPHQSSSPSR 对镓表现出最高的亲和力。肽 NYLPHQSSSPSR 的计算建模用于确定相互作用参数并解释可能的结合机制。此外，肽被固定在聚苯乙烯珠上。因此，我们创建了一种新型的、异常坚固的基于肽的材料，用于从水溶液中吸附镓。
