Gevorkian G A, Simonian A A, Voskanian L O
Ukr Biokhim Zh (1978). 1988 Jan-Feb;60(1):19-23.
Proteins of inner mitochondrial membranes of the albino rat myocardium during postnatal development of 1, 3 and 6 months old animals were electrophoretically separated in 10% polyacrylamide gel. The rate of 14C-amino acids incorporation into examined proteins was determined in vitro. Specific radioactivity of the total mitochondrial fraction decreased in the course of the postnatal development. That of outer membranes remained unchanged, though it sharply increased in inner membranes of mature animals as compared with animals aged one month. Levels of radioactive precursor incorporation in separate protein fractions of inner membranes of the myocardium mitochondria were estimated.
在出生后1个月、3个月和6个月大的白化大鼠心肌线粒体内膜蛋白质,在10%聚丙烯酰胺凝胶中进行了电泳分离。体外测定了14C-氨基酸掺入所检测蛋白质的速率。在出生后发育过程中,线粒体总组分的比放射性降低。外膜的比放射性保持不变,而与1个月龄的动物相比,成熟动物内膜的比放射性急剧增加。估计了心肌线粒体内膜各蛋白质组分中放射性前体的掺入水平。