Kopteva L A, Zhevoliukova L I, Grigor'eva E A
Biull Eksp Biol Med. 1975 Dec;80(12):90-3.
Two fractions of the inner membranes of the mitochondria were isolated from rat myocardium: complex-the inner membranes-matrix (fraction A); the aggregates of the inner membranes deproved of the matrix (fraction B). Both fractions of the membranes effectuated the incorporation of the radioactive amino acids with the same activity. With the aid of gel electrophoresis the amount of the protein components of A and B fractions was found to bary from 20 to 30. The mol wts were from 10000 to 200000. Disseminated myocardial necrosis produced by novodrine injection based on the ischemic state of the heart led in two days to a considerable decrease in the protein synthesizing activity of fractions A and B. The protein profiles and the mol wt of the membrane protein components displayed no marked changes.
复合物——内膜-基质（组分A）；不含基质的内膜聚集体（组分B）。这两个膜组分以相同活性实现放射性氨基酸的掺入。借助凝胶电泳发现，A和B组分的蛋白质成分数量在20至30之间变化。分子量在10000至200000之间。基于心脏缺血状态，注射新福林导致的弥漫性心肌坏死在两天内使组分A和B的蛋白质合成活性显著降低。膜蛋白成分的蛋白质谱和分子量没有明显变化。
