Graduate School of Agriculture, University of Miyazaki, Miyazaki, Japan.
Ruminants Feeding Standard Research and Development Center, Khon Kaen, Thailand.
Anim Sci J. 2021 Jan-Dec;92(1):e13531. doi: 10.1111/asj.13531.
The purpose of this study was to investigate the effects of feeding Bacillus subtilis on rumen fermentation, blood metabolites, nutrient digestibility, and energy and nitrogen balances in non-lactating crossbred (Holstein-Friesian × Bos indicus) cows. Four cows were assigned to the control and B. subtilis diets in a crossover design, and respiratory and metabolic experiments were conducted. For the B. subtilis diet, B. subtilis DSM15544 spores were added at the rate of 1.0 × 10 CFU/head/day to the control diet. At 4 hr after feeding, cows fed the B. subtilis diet had increased levels of i-butyric acid in the rumen fluid and tended to have lower concentrations of plasma non-esterified fatty acids when compared with cows fed the control diet. This suggests that feeding B. subtilis could improve energy efficiency. However, there was no effect on energy retention in this study. Although there were no effects on nutrient digestibility, nitrogen balance, or methane production, heat production was significantly higher in cows fed the B. subtilis diet than in those fed the control diet.
本研究旨在探讨饲用枯草芽孢杆菌对泌乳前期杂交（荷斯坦-弗里生牛×印度瘤牛）奶牛瘤胃发酵、血液代谢物、养分消化率以及能量和氮平衡的影响。采用交叉设计，将 4 头奶牛分为对照组和枯草芽孢杆菌组，进行呼吸和代谢试验。在枯草芽孢杆菌组的日粮中，枯草芽孢杆菌 DSM15544 孢子的添加量为 1.0×10 CFU/头/天，添加到对照组日粮中。在饲喂后 4 小时，与对照组相比，饲喂枯草芽孢杆菌组奶牛瘤胃液中 i-丁酸的水平增加，且血浆非酯化脂肪酸的浓度趋于降低。这表明饲用枯草芽孢杆菌可以提高能量效率。然而，本研究中没有观察到对能量保留的影响。虽然对养分消化率、氮平衡或甲烷生成没有影响，但饲喂枯草芽孢杆菌组奶牛的产热量显著高于对照组。
