Meenongyai Watcharawit, Rasri Kampanat, Rodjapot Sitthisak, Duangphayap Trirat, Khejornsart Pichad, Wongpanit Kannika, Phongkaew Piyamas, Bashar Abul, Islam Zakirul
Department of Agriculture and Resources, Faculty of Natural Resources and Agro-Industry, Kasetsart University Chalermphrakiat Sakon Nakhon Province Campus, Sakon Nakhon, 47000, Thailand.
Department of Animal Breeding and Genetics, Bangladesh Agricultural University, Mymensingh, 2202, Bangladesh.
Trop Anim Health Prod. 2023 Feb 7;55(2):69. doi: 10.1007/s11250-023-03499-2.
This study aimed to determine the effects of coated cysteamine hydrochloride (CSH) and probiotics (PB) supplemented alone or in combination on feed intake, digestibility, ruminal fermentation, and blood metabolites of heifer beef cattle. Sixteen heifers (body weight = 210 ± 41 kg; age = 9 ± 2 months) were assigned according to a randomized complete block design in a 2 × 2 factorial arrangement. All animals were fed the basal diet, which contained an 82:17 concentrate-to-forage ratio, and the forage source was rice straw. The treatments were as follows: (1) 0% PB + 0 g/d CSH, (2) 0.1% PB + 0 g/d CSH, (3) 0% PB + 20 g/d CSH, and (4) 0.1% PB + 20 g/d CSH. The main effect of CSH supplementation has been found to improve feed intake (P < 0.05). There were no treatment interactions with nutrient digestibility or rumen fermentation parameters. Supplementation of CSH did not affect any of the variables evaluated, while probiotics supplementation increased DM digestibility due to the increases in CP and fiber fraction digestibility. Compared to controls and CSH, at 16 h post-feeding, heifers receiving probiotics tended (P = 0.07) to show 17% greater ruminal NH3-N concentration, but this effect was not evident at 2 h post-feeding. However, the main effects of probiotic supplementation showed a tendency to increase the number of total bacteria and fungal zoospores in the rumen at 2 h post-feeding. The blood triglyceride (BTG) concentration of heifers fed a diet supplemented with 20 g/d CSH and 0.1% probiotics was found to be greater than those fed CSH alone (P < 0.1) at 16 h post-feeding, and then, there were greater BTG concentrations than other treatments (P < 0.05) at 2 h post-feeding. In conclusion, the combination of CSH and PB did not potentiate the effects of probiotics on digestibility and rumen fermentation and had minimal effects on blood parameters.
本研究旨在确定单独或联合添加包膜盐酸半胱胺（CSH）和益生菌（PB）对小母牛育肥牛采食量、消化率、瘤胃发酵和血液代谢物的影响。16头小母牛（体重=210±41千克；年龄=9±2个月）按照随机完全区组设计，采用2×2析因排列方式进行分组。所有动物均饲喂基础日粮，其精粗比为82:17，粗饲料来源为稻草。处理方式如下：（1）0%PB + 0克/天CSH，（2）0.1%PB + 0克/天CSH，（3）0%PB + 20克/天CSH，以及（4）0.1%PB + 20克/天CSH。已发现添加CSH的主要作用是提高采食量（P<0.05）。在营养物质消化率或瘤胃发酵参数方面不存在处理间交互作用。添加CSH对所评估的任何变量均无影响，而添加益生菌由于粗蛋白和纤维组分消化率的提高而增加了干物质消化率。与对照组和CSH组相比，在采食后16小时，接受益生菌的小母牛瘤胃NH₃-N浓度有高出17%的趋势（P = 0.07），但在采食后2小时这种效应不明显。然而，添加益生菌的主要作用表现为在采食后2小时有增加瘤胃总细菌和真菌游动孢子数量的趋势。发现采食添加20克/天CSH和0.1%益生菌日粮的小母牛在采食后16小时血液甘油三酯（BTG）浓度高于仅采食CSH的小母牛（P<0.1），并且在采食后2小时其BTG浓度高于其他处理组（P<0.05）。总之，CSH和PB的组合并未增强益生菌对消化率和瘤胃发酵的作用，且对血液参数影响极小。
