Shi Yuyan, Fu Xiaoyi, Yin Yao, Peng Fangqi, Yin Xia, Ke Guoliang, Zhang Xiaobing
Molecular Science and Biomedicine Laboratory, State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha, 410082, P. R. China.
Chem Asian J. 2021 Apr 19;16(8):857-867. doi: 10.1002/asia.202100043. Epub 2021 Mar 18.
Clustered regularly interspaced short palindromic repeats (CRISPR) is a promising technology in the biological world. As one of the CRISPR-associated (Cas) proteins, Cas12a is an RNA-guided nuclease in the type V CRISPR-Cas system, which has been a robust tool for gene editing. In addition, due to the discovery of target-binding-induced indiscriminate single-stranded DNase activity of Cas12a, CRISPR-Cas12a also exhibits great promise in biosensing. This minireview not only gives a brief introduction to the mechanism of CRISPR-Cas12a but also highlights the recent developments and applications in biosensing and gene regulation. Finally, future prospects of the CRISPR-Cas12a system are also discussed. We expect this minireview will inspire innovative work on the CRISPR-Cas12a system by making full use of its features and advantages.
成簇规律间隔短回文重复序列（CRISPR）是生物界一项很有前景的技术。作为CRISPR相关（Cas）蛋白之一，Cas12a是V型CRISPR-Cas系统中的一种RNA引导核酸酶，它一直是一种强大的基因编辑工具。此外，由于发现了Cas12a的靶标结合诱导的非特异性单链DNA酶活性，CRISPR-Cas12a在生物传感方面也展现出巨大潜力。这篇综述不仅简要介绍了CRISPR-Cas12a的作用机制，还重点介绍了其在生物传感和基因调控方面的最新进展及应用。最后，还讨论了CRISPR-Cas12a系统的未来前景。我们希望这篇综述能通过充分利用CRISPR-Cas12a系统的特性和优势，激发有关该系统的创新性研究工作。
