四种陆栖繁殖无尾两栖动物中的激素诱导排卵及人工授精

Hormone-induced ovulation and artificial fertilisation in four terrestrial-breeding anurans.

作者信息

Silla Aimee J, Byrne Phillip G

机构信息

School of Earth, Atmospheric and Life Sciences, University of Wollongong, Wollongong, NSW 2522, Australia; and School of Biological Sciences, University of Western Australia, Stirling Highway, Nedlands, WA 6009, Australia; and Corresponding author.

School of Earth, Atmospheric and Life Sciences, University of Wollongong, Wollongong, NSW 2522, Australia.

出版信息

Reprod Fertil Dev. 2021 Mar;33:615-618. doi: 10.1071/RD20243.

DOI:10.1071/RD20243

PMID:33640035

Abstract

The development and application of reproductive technologies has great potential to enhance the conservation management of threatened amphibians globally. The present study quantified the efficacy of protocols previously developed for Pseudophryne guentheri for hormonally inducing egg release and artificial fertilisation in three additional terrestrial-breeding species of Australian ground frog; namely Pseudophryne bibronii , Pseudophryne coriacea and Heleioporus eyrei . Females of each species were administered a priming dose of 0.4µg g-1 of gonadotropin-releasing hormone analogue (GnRHa), followed by an ovulatory dose of 2µg g-1 GnRHa. Egg release was achieved in all species (response 94.7-100%), but early embryo survival differed significantly, ranging from 23.8% to 89.3%. Overall, the protocols were considered effective in P. guentheri , P. bibronii and P. coriacea , but further protocol refinement is required for H. eyrei .

摘要

生殖技术的发展与应用在加强全球濒危两栖动物的保护管理方面具有巨大潜力。本研究量化了先前为冈氏拟蟾开发的方案在另外三种澳大利亚陆栖蛙类（即比氏拟蟾、革拟蟾和艾氏盔蟾）中诱导产卵和人工授精的效果。给每个物种的雌性注射0.4μg g-1的促性腺激素释放激素类似物（GnRHa）作为启动剂量，随后注射2μg g-1 GnRHa作为排卵剂量。所有物种均实现了产卵（反应率为94.7 - 100%），但早期胚胎存活率差异显著，范围为23.8%至89.3%。总体而言，这些方案在冈氏拟蟾、比氏拟蟾和革拟蟾中被认为是有效的，但艾氏盔蟾需要进一步完善方案。