Chen Jie, Xiao Xiyan, He Shan, Qiao Yi, Ma Shuwei
Department of Otorhinolaryngology - Head and Neck Surgery, Shanghai Children's Medical Center, Shanghai Jiaotong University School of Medicine, Shanghai, PR China.
Allergol Immunopathol (Madr). 2021 Mar 1;49(2):94-103. doi: 10.15586/aei.v49i2.33. eCollection 2021.
Emerging evidence shows that circular RNAs (circRNAs) participate in the pathogenesis of multiple immune diseases. However, few studies have focused on the mechanisms of circRNAs involved in allergic rhinitis (AR).
This study performed an RNA sequence (RNA-seq) profiling to identify the expression of circRNAs in nasal mucosa from ovalbumin-induced AR murine models and normal controls. Quantitative real-time reverse transcriptase polymerase chain reaction (qRT-PCR) was then conducted to validate the differential expression of circRNAs. Bioinformatics analysis was applied to demonstrate the biological functions of the dysregulated circRNAs.
A total of 86 distinct circRNA candidates were sequenced, of which 51 were upregulated and 35 were downregulated. The T cell receptor, B cell receptor, and calcium signaling pathways may be involved in the pathology of AR. Furthermore, a circRNA-miRNA interaction network was constructed via miRNA response elements analysis. Some circRNAs were correlated with miRNAs that are involved in T cell polarization and activation, thereby highlighting their potential role in the pathogenesis of AR.
This study demonstrates a number of aberrantly expressed circRNAs related to AR, and offers a novel perspective into AR pathogenesis and future therapeutic strategies.
新出现的证据表明，环状RNA（circRNA）参与多种免疫疾病的发病机制。然而，很少有研究关注circRNA参与变应性鼻炎（AR）的机制。
本研究进行了RNA测序（RNA-seq）分析，以鉴定卵清蛋白诱导的AR小鼠模型和正常对照的鼻黏膜中circRNA的表达。随后进行定量实时逆转录聚合酶链反应（qRT-PCR）以验证circRNA的差异表达。应用生物信息学分析来证明失调的circRNA的生物学功能。
共测序了86个不同的circRNA候选物，其中51个上调，35个下调。T细胞受体、B细胞受体和钙信号通路可能参与AR的病理过程。此外，通过miRNA反应元件分析构建了circRNA-miRNA相互作用网络。一些circRNA与参与T细胞极化和激活的miRNA相关，从而突出了它们在AR发病机制中的潜在作用。
本研究证明了一些与AR相关的异常表达的circRNA，并为AR发病机制和未来治疗策略提供了新的视角。
