人精子中类人绒毛膜促性腺激素物质的免疫检测

Immunodetection of a human chorionic gonadotropin-like substance in human sperm.

作者信息

Asch R H, Fernandez E O, Pauerstein C J

出版信息

Fertil Steril. 1977 Nov;28(11):1258-62. doi: 10.1016/s0015-0282(16)42928-6.

Abstract

The presence of a human chorionic gonadotropin (hCG)-like substance in human spermatozoa is reported. A highly sensitive immunocytochemical procedure was utilized (double-antibody immunofluorescence technique). Rabbit anti-hCG or rabbit anti-hCG beta-subunit was used as the first antibody. A positive fluorescence reaction was found in all human specimens analyzed and in positive controls (choriocarcinoma cells). No fluorescence was detected in other species studied (sheep, pig, goat, horse, bull, and guinea pig), nor in the negative controls. These findings open a new research area on the physiologic role of this hCG-like substance in human reproduction.

摘要

据报道，人类精子中存在一种人绒毛膜促性腺激素（hCG）样物质。采用了一种高度灵敏的免疫细胞化学方法（双抗体免疫荧光技术）。兔抗hCG或兔抗hCGβ亚基用作一抗。在所分析的所有人类标本以及阳性对照（绒毛膜癌细胞）中均发现了阳性荧光反应。在研究的其他物种（绵羊、猪、山羊、马、公牛和豚鼠）以及阴性对照中均未检测到荧光。这些发现为这种hCG样物质在人类生殖中的生理作用开辟了一个新的研究领域。