牛角膜上皮中的细胞外液量

Extracellular volume in the bovine corneal epithelium.

作者信息

Midelfart A

机构信息

Department of Ophthalmology, Faculty of Medicine, University of Trondheim, Norway.

出版信息

Acta Ophthalmol (Copenh). 1988 Feb;66(1):93-8. doi: 10.1111/j.1755-3768.1988.tb08541.x.

DOI:10.1111/j.1755-3768.1988.tb08541.x

PMID:3364175

Abstract

Three different substances, [14C]inulin, [14C]polyethylenglycol (PEG, molecular weight 4000) and [3H]sucrose have been examined for suitability as markers for determination of the extracellular volume (ECV) in the bovine corneal epithelium and stroma. In both tissues, sucrose was the best marker reaching equilibrium of distribution within 90 min. Inulin and 4000 PEG have been found to be unacceptable because of a slow rate of penetration of the ECV. In addition, both inulin and 4000 PEG gave considerably lower values for the ECV than sucrose. Using the value for ECV determined with sucrose as a marker in the bovine corneal epithelium, i.e. 21.6 +/- 1.4% of tissue water, and the results obtained previously by the chemical analysis of this tissue (Midelfart 1987) the intracellular concentrations of 34.4 +/- 8.6 mmol Na/kg H2O and 154.3 +/- 11.1 mmol K/kg H2O were determined. These results were compared with the results obtained by other methods in corneae from other species.

摘要

已对三种不同物质，即[¹⁴C]菊粉、[¹⁴C]聚乙二醇（PEG，分子量4000）和[³H]蔗糖，作为测定牛角膜上皮和基质细胞外液量（ECV）的标志物的适用性进行了研究。在这两种组织中，蔗糖是最佳标志物，在90分钟内达到分布平衡。由于菊粉和4000 PEG穿透ECV的速率较慢，因此发现它们不适用。此外，菊粉和4000 PEG得出的ECV值比蔗糖低得多。以蔗糖作为标志物测定牛角膜上皮的ECV值，即占组织水的21.6±1.4%，并结合此前对该组织进行化学分析所得结果（米德尔法特，1987年），确定了细胞内浓度为34.4±8.6 mmol Na/kg H₂O和154.3±11.1 mmol K/kg H₂O。将这些结果与通过其他方法在其他物种角膜中获得的结果进行了比较。