氨氯吡咪对牛角膜上皮中钠、钾和水的作用。

The effect of amiloride on Na, K and water in bovine corneal epithelium.

作者信息

Midelfart A

机构信息

Department of Ophthalmology, Faculty of Medicine, University of Trondheim, Norway.

出版信息

Exp Eye Res. 1987 Dec;45(6):751-62. doi: 10.1016/s0014-4835(87)80092-1.

DOI:10.1016/s0014-4835(87)80092-1

PMID:3428398

Abstract

The Na and K concentrations of bovine corneal epithelium and stroma were determined by chemical analysis. The effect of ambient Na concentration on these concentrations was evaluated. In the epithelium, approx. 10 mmol Na kg-1 H2O entered the cells from the tear side of the cornea. An inhibition of Na entry at the apical membrane by 5 x 10(-5) mol l-1 amiloride resulted in a loss of this amount of Na from the cells, accompanied by a loss of water. A linear relationship between the Na concentration in the stromal tissue and Na concentration on the on the aqueous side of the cornea was observed.

摘要

通过化学分析测定了牛角膜上皮和基质中的钠和钾浓度。评估了环境钠浓度对这些浓度的影响。在上皮中，约10 mmol Na kg-1 H2O从角膜的泪液侧进入细胞。5×10(-5) mol l-1的氨氯吡咪对顶端膜处钠进入的抑制导致细胞中该量的钠流失，并伴有水分流失。观察到基质组织中的钠浓度与角膜房水侧的钠浓度之间呈线性关系。